检测大鼠潜伏性基尔汉姆病毒感染的灵敏方法的开发。
Development of sensitive methods for detection of latent Kilham virus infection of rats.
作者信息
Shalunova N V, Dzagurov S G, Gavrilov K I, Petruchuk E M, Gusarevich E A, Uskov V N, Babanova T A
出版信息
Acta Virol. 1975 Jul;19(4):354-8.
The developed system of procedures, including preliminary selection of rats according to the presence of 19 S antibody in the blood serum, and the use of the most sensitive methods has increased considerably the incidence of detection of Kilham virus (KV) in organs of latently infected rats. To isolate and establish KV strains from the organs of rats in which the specific antigen was detected by immunofluorescence, at least 5-9 passages in rat embryo cell cultures or in newborn Syrian hamsters were required.
所开发的程序系统，包括根据血清中19S抗体的存在对大鼠进行初步筛选，以及使用最敏感的方法，显著提高了在潜伏感染大鼠器官中检测基尔汉姆病毒（KV）的发生率。为了从通过免疫荧光检测到特异性抗原的大鼠器官中分离并建立KV毒株，需要在大鼠胚胎细胞培养物或新生叙利亚仓鼠中至少传代5 - 9次。
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