Liu Yimei, Wang Ke, Liu Zhen, Luo Kun, Chen Shilin, Chen Keli
Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, P.R. China.
Pharmacogn Mag. 2013 Oct;9(36):331-7. doi: 10.4103/0973-1296.117829.
Ardisia is a group of famous herbs in China, which has been used as medical plants for more than 900 years. However, the species from the genus are so analogous that it is difficult to discriminate them just by morphological characteristics. DNA barcoding is a new technique that uses a short and standard fragment of DNA sequences to identify species.
Choose a suitable DNA marker to authenticate Ardisia species.
Four markers (psbA-trnH, internal transcribed spacer 2 [ITS2], rbcL, matK) were tested on 54 samples of 24 species from genus Ardisia. The success rates of polymerase chain reaction amplification and sequencing, differential intra- and inter-specific divergences, DNA barcoding gap and identification efficiency were used to evaluate the discrimination ability.
The results indicate that matK has the highest interspecific divergence and significant differences between inter- and intra-specific divergences, whereas psbA-trnH, ITS2 and rbcL have much lower divergence values. Matk possessed the highest species identification efficiency at 98.1% by basic local alignment search tool 1 [BLAST1], method and 91.7% by the nearest distance method.
The matK region is a promising DNA barcode for the genus Ardisia.
紫金牛属植物是中国著名的药用植物类群，作为药用植物已有900多年的历史。然而，该属植物种类相似，仅通过形态特征难以区分。DNA条形码是一种利用短的标准DNA序列片段来鉴定物种的新技术。
选择合适的DNA标记物对紫金牛属植物进行鉴定。
对紫金牛属24个物种的54个样本测试了4个标记物（psbA-trnH、内转录间隔区2 [ITS2]、rbcL、matK）。采用聚合酶链反应扩增和测序成功率、种内和种间差异、DNA条形码间隙和鉴定效率来评估区分能力。
结果表明，matK具有最高的种间差异，种间和种内差异之间存在显著差异，而psbA-trnH、ITS2和rbcL的差异值则低得多。通过基本局部比对搜索工具1 [BLAST1]方法，matK的物种鉴定效率最高，为98.1%，通过最近距离法为91.7%。
matK区域是紫金牛属植物一种很有前景的DNA条形码。
