Department of Food Science, Liaoning University, Shenyang 110036, China.
Food Chem. 2014 Feb 15;145:335-41. doi: 10.1016/j.foodchem.2013.08.036. Epub 2013 Aug 16.
To expand application of hawthorn (Crataegus pinnatifida Bge) fruit, the antioxidant and anti-lipidemic effects of haw pectin penta-oligogalacturonide (HPPS) prepared from hawthorn fruit were investigated in vitro and in mice. HPPS exhibited concentration-dependent scavenging activities against superoxide anion, hydroxyl and DPPH radicals. Additionally, HPPS supplementation significantly enhanced the antioxidant enzyme activities of superoxide dismutase, catalase, glutathione peroxidase, increased the total antioxidant capacity and the levels of glutathione, but lowered the malondialdehyde content in the liver of high-fat fed mice. Furthermore, HPPS significantly decreased the TG levels, the activity and the mRNA and protein levels of glycerol 3-phosphate acyltransferase (GPAT) and phosphatidate phosphohydrolase (PAP) in mice livers. Moreover, liver steatosis of mice associated with diffuse hepatocyte ballooning induced by a high-fat diet was markedly improved by a dose of 300 mg/kg HPPS-consumption. The results revealed that HPPS might be applicable as a dietary supplement for the prevention of fatty liver and oxidative damage.
为拓展山楂（Crataegus pinnatifida Bge）果实的应用范围，本研究以山楂果实为原料制备得到的果胶五寡半乳糖醛酸（HPPS），并考察其体外和体内的抗氧化及抗脂质过氧化作用。结果表明，HPPS 对超氧阴离子自由基、羟自由基和 DPPH 自由基均具有浓度依赖性的清除作用。此外，HPPS 可显著提高高脂膳食小鼠肝脏中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性，增加总抗氧化能力和还原型谷胱甘肽水平，降低丙二醛含量。HPPS 还可显著降低 TG 水平以及甘油 3-磷酸酰基转移酶（GPAT）和磷酸酶（PAP）的活性和 mRNA 及蛋白表达水平。此外，HPPS 还可明显改善由高脂膳食引起的小鼠肝脏脂肪变性和弥漫性气球样变。综上所述，HPPS 可作为一种膳食补充剂，用于预防脂肪肝和氧化损伤。
