Shao Feng, Gu Lifei, Chen Huijuan, Liu Ronghua, Huang Huilian, Ren Gang
Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Deptment of Pharmacy, The People's Hospital of Bozhou, Anhui, China.
Pharmacogn Mag. 2016 Jan-Mar;12(45):64-9. doi: 10.4103/0973-1296.176049.
Hawthorn (Crataegus pinnatifida) is a Chinese medicinal plant traditionally used in the treatment of hyperlipidemia. Recently, studies indicated free radical scavenging was one of the major pathways to alleviate hyperlipidemia. Moreover, hawthorn fruit is a rich source of phenols, which quench free radical and attenuate hyperlipidemia. However, the phenols vary with processing methods, especially solvent type.
Our aim was to compare hypolipidemic and antioxidant effects of aqueous and ethanol extracts of hawthorn fruit in hyperlipidemia rats.
After a 4-week treatment of high-fat emulsion, lipid profile levels and antioxidant levels of two extracts were determined using commercial analysis. Total phenols content in the extract of hawthorn fruit was determined colorimetrically by the Folin-Ciocalteu method.
Both ethanol and aqueous extracts of hawthorn fruit possessed hypolipidemic and antioxidant activities. Simultaneously, stronger activities were observed in ethanol extract. Besides, total phenols content in ethanol extract from the same quality of hawthorn fruit was 3.9 times more than that in aqueous extract.
The obvious difference of hypolipidemic and antioxidant effects between ethanol extract and aqueous extract of hawthorn fruit was probably due to the presence of total phenols content, under the influence of extraction solvent.
Ethanol extract of hawthorn fruit exhibited more favorable hypolipidemic and antioxidant effects than aqueous extract. The higher effects could be due to the higher content of total phenols that varies with extraction solvent. Abbreviations used: TC: Total cholesterol, TG: Triglyceride, LDL-C: Low-density lipoprotein cholesterol, HDL-C: High-density lipoprotein cholesterol, GSH-Px: Glutathione peroxidase, SOD: Superoxide dismutase, MDA: Malondialdehyde, CAT: Catalase, NO: Nitric oxide, NOS: Nitric oxide synthase, SR-BI: Scavenger receptor Class B Type I.
山楂（山里红）是一种传统上用于治疗高脂血症的中国药用植物。最近，研究表明清除自由基是缓解高脂血症的主要途径之一。此外，山楂果实富含酚类物质，这些酚类物质可淬灭自由基并减轻高脂血症。然而，酚类物质会因加工方法而异，尤其是溶剂类型。
我们的目的是比较山楂果实水提取物和乙醇提取物对高脂血症大鼠的降血脂和抗氧化作用。
在给予高脂乳剂4周后，使用商业分析方法测定两种提取物的血脂水平和抗氧化水平。采用福林-酚法比色法测定山楂果实提取物中的总酚含量。
山楂果实的乙醇提取物和水提取物均具有降血脂和抗氧化活性。同时，乙醇提取物的活性更强。此外，相同质量山楂果实的乙醇提取物中的总酚含量是水提取物的3.9倍。
山楂果实乙醇提取物和水提取物在降血脂和抗氧化作用上的明显差异可能是由于在提取溶剂的影响下总酚含量的不同。
山楂果实乙醇提取物比水提取物表现出更有利的降血脂和抗氧化作用。较高的效果可能归因于随提取溶剂变化的较高总酚含量。使用的缩写：TC：总胆固醇，TG：甘油三酯，LDL-C：低密度脂蛋白胆固醇，HDL-C：高密度脂蛋白胆固醇，GSH-Px：谷胱甘肽过氧化物酶，SOD：超氧化物歧化酶，MDA：丙二醛，CAT：过氧化氢酶，NO：一氧化氮，NOS：一氧化氮合酶，SR-BI：B类清道夫受体I型
