Institute of Biotechnology, Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, 100000, Vietnam.
Institute of Genome Research, Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
Biotechnol Lett. 2020 Jun;42(6):987-995. doi: 10.1007/s10529-020-02836-x. Epub 2020 Feb 12.
The isolation, identification and characterization of a novel huperzine A (HupA)-producing fungal strain Rsp5.2 isolated from Huperzia serrata (Thunb. ex Murray) Trev. in Vietnam. The fifty-eight endophytic fungi were recovered from roots of natural H. serrata in Lao Cai province of northern Vietnam and screened for HupA-producing by thin-layer chromatography (TLC). The only one of the 58 strains, Rsp5.2, could produce HupA. The amount of HupA produced by Rsp5.2 was quantified to be 19.45 µg g dried mycelium by high-performance liquid chromatography (HPLC). Acetylcholine esterase (AchE) inhibition (IC) of the crude HupA extract from Rsp5.2 fermentation broth was 2.849 ± 0.0026 µg mL. The fungus was identified as Fusarium sp. Rsp5.2 by morphological characteristics and Internal Transcribed Spacer (ITS) sequences. This is the first report of Fusarium sp. as a HupA-producing endophyte isolated from H. serrata.
从越南的蛇足石杉（Thunb. ex Murray） Trev. 中分离、鉴定和表征一种新型石杉碱甲（HupA）产生真菌菌株 Rsp5.2。从越南北部老街省天然蛇足石杉的根部回收了 58 株内生真菌，并通过薄层层析（TLC）筛选 HupA 产生菌。在 58 株菌株中，只有一株，Rsp5.2，能够产生 HupA。通过高效液相色谱（HPLC）定量 Rsp5.2 产生的 HupA 量为 19.45 µg g 干燥菌丝体。来自 Rsp5.2 发酵液的粗 HupA 提取物对乙酰胆碱酯酶（AchE）的抑制（IC）为 2.849 ± 0.0026 µg mL。通过形态特征和内部转录间隔区（ITS）序列，该真菌被鉴定为尖孢镰刀菌。这是首次报道从蛇足石杉中分离出尖孢镰刀菌作为产生石杉碱甲的内生真菌。
