Avtushenko S S, Smirnova L F
Mikrobiologiia. 1985 May-Jun;54(3):398-401.
The object of this work was to study how the synthesis of protein, RNA and DNA in Escherichia coli M17 and its viability were influenced by chloramphenicol (50 and 300 micrograms/ml) an inhibitor of protein biosynthesis, and sodium azide (200 and 2000 microM) and aminazine (50 micrograms/ml), inhibitors of respiration. The exposed were inhibitors with the bacteria for 60 min at room temperature and for 1-4 months at -10 degrees C. The inhibition of the E. coli viability by chloramphenicol was shown to be reversible. The respiration inhibitors stabilized its viability upon storage at -10 degrees C for one month. The inhibitors were found to produce a different effect on the synthesis of RNA and protein in E. coli. The rates of DNA synthesis hardly changed. No correlation was established between changes in the synthesis of protein and nucleic acids by E. coli after the action of the inhibitors and its viability.
这项工作的目的是研究蛋白质生物合成抑制剂氯霉素（50和300微克/毫升）、呼吸抑制剂叠氮化钠（200和2000微摩尔）及氯丙嗪（50微克/毫升）如何影响大肠杆菌M17中蛋白质、RNA和DNA的合成及其活力。将抑制剂与细菌在室温下接触60分钟，并在-10℃下接触1至4个月。结果表明，氯霉素对大肠杆菌活力的抑制作用是可逆的。呼吸抑制剂在-10℃储存一个月时可稳定其活力。发现这些抑制剂对大肠杆菌中RNA和蛋白质的合成产生不同影响。DNA合成速率几乎没有变化。在抑制剂作用后，大肠杆菌蛋白质和核酸合成的变化与其活力之间未建立相关性。
