Ramalingam V, Rajaram R, PremKumar C, Santhanam P, Dhinesh P, Vinothkumar S, Kaleshkumar K
DNA Barcoding and Marine Genomics Laboratory, Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, India.
J Basic Microbiol. 2014 Sep;54(9):928-36. doi: 10.1002/jobm.201300514. Epub 2013 Oct 18.
Pseudomonas aeruginosa (JQ989348) was isolated from deep sea water sample and used for synthesis of silver nanoparticles (AgNPs). AgNPs were confirmed by analyzing surface plasmon resonance using UV-visible spectrophotometer at 420 nm. Further scanning electron microscope analysis confirmed the range of particle size between 13 and 76 nm and XRD pattern authorizes the anisotropic crystalline nature of AgNPs. Fourier transform infrared spectrum endorsed the presence of high amount of proteins and other secondary metabolites in synthesized AgNPs influence the reduction process and stabilization of nanoparticles. The inhibitory activity of AgNPs was tested against human pathogens showed high activity against Eschericia coli, Vibrio cholerae, Aeromonas sp., and Cornebacterium sp. demonstrating its antimicrobial value against pathogenic diseases. Additionally, biologically synthesized AgNPs have notable anti-biofilm activity against primary biofilm forming bacteria P. aeruginosa and Staphylococcus aureus. The MTT assay method was evaluated using human cervical cancer cells exposed the AgNPs have excellent cytotoxic activity.
铜绿假单胞菌（JQ989348）从深海水样中分离出来，用于合成银纳米颗粒（AgNPs）。通过使用紫外可见分光光度计在420nm处分析表面等离子体共振来确认AgNPs。进一步的扫描电子显微镜分析证实粒径范围在13至76nm之间，XRD图谱证实了AgNPs的各向异性晶体性质。傅里叶变换红外光谱证实合成的AgNPs中存在大量蛋白质和其他次生代谢产物，影响了纳米颗粒的还原过程和稳定性。测试了AgNPs对人类病原体的抑制活性，结果表明其对大肠杆菌、霍乱弧菌、气单胞菌属和棒状杆菌属具有高活性，证明了其对致病性疾病的抗菌价值。此外，生物合成的AgNPs对形成初始生物膜的细菌铜绿假单胞菌和金黄色葡萄球菌具有显著的抗生物膜活性。使用暴露于AgNPs的人宫颈癌细胞评估MTT测定法，结果表明其具有优异的细胞毒性活性。
