Sayed Alaa El-Din H, Mohamed Nadia H, Ismail Mady A, Abdel-Mageed Wael M, Shoreit Ahmed A M
Department of Zoology, Faculty of Science, Assiut University, Assiut, Egypt.
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt; Department of Biology, Faculty of Science and Art, Samtah, Jazan University, Saudi Arabia.
Ecotoxicol Environ Saf. 2016 Jun;128:189-94. doi: 10.1016/j.ecoenv.2016.02.023. Epub 2016 Mar 3.
Calotropis procera L. is known as medicinal plant. The Phytochemical analyzes of its latex revealed that it possessed antioxidants, namely terpenes, phenolic compounds and cardenolides, flavonoids and saponins, while tannins, alkaloids and resin were absent in moderate to high concentration. In the present study, the role of latex of Calotropis procera as antioxidant and antiapoptotic was reported. To carry out this aim, fishes were exposed to 100 µg l(-1) 4-nonylphenol as chemical pollutant. The enzymes, superoxidase dismutase, catalase, acetlycholinstrase (AchE), glutathione s-transferase, cortisol, G6PDH) and apoptotic cells increased significantly (p<0.05) accompanied by irregular disturbance of (Na(+), K(+)) ions in the presence of 4-nonylphenol. On the other hand, these enzymes, ions, and apoptotic cells decreased normally and significantly (p<0.05) in the presence of latex. Total phenol content, total capacity antioxidant, reducing power decrease significantly (p<0.05) in the presence of 4-nonylphenol and increase normally in the presence of latex. Latex was used for the first time to protect catfish after 4-nonylphenol exposure. Our study confirms that crude latex of Calotropis procera possessed antioxidant and antiapoptotic activities against the toxicity of 4-Nonylphenol.
牛角瓜是一种药用植物。对其乳汁的植物化学分析表明，它含有抗氧化剂，即萜类、酚类化合物、强心苷、黄酮类化合物和皂苷，而单宁、生物碱和树脂的含量则处于中低水平。在本研究中，报道了牛角瓜乳汁作为抗氧化剂和抗凋亡剂的作用。为了实现这一目标，将鱼类暴露于100μg l(-1)的4-壬基酚作为化学污染物。在4-壬基酚存在的情况下，超氧化物歧化酶、过氧化氢酶、乙酰胆碱酯酶（AchE）、谷胱甘肽S-转移酶、皮质醇、G6PDH等酶以及凋亡细胞显著增加（p<0.05），同时伴有（Na(+)，K(+)）离子的不规则紊乱。另一方面，在乳汁存在的情况下，这些酶、离子和凋亡细胞正常且显著减少（p<0.05）。在4-壬基酚存在的情况下，总酚含量、总抗氧化能力、还原能力显著降低（p<0.05），而在乳汁存在的情况下则正常增加。首次使用乳汁在4-壬基酚暴露后保护鲶鱼。我们的研究证实，牛角瓜粗乳汁对4-壬基酚的毒性具有抗氧化和抗凋亡活性。
