Bosman A B, Wagenaar J A, Stegeman J A, Vernooij J C M, Mevius D J
Faculty of Veterinary Medicine,Utrecht University,Department of Infectious Diseases and Immunology, Utrecht,The Netherlands.
Faculty of Veterinary Medicine,Utrecht University,Department of Farm Animal Health,The Netherlands.
Epidemiol Infect. 2014 Sep;142(9):1893-904. doi: 10.1017/S0950268813002665. Epub 2013 Oct 24.
The aim of this study was to determine the association between farm management factors, including antimicrobial drug usage, and resistance in commensal Escherichia coli isolates from the faeces of white veal calves. Ninety E. coli isolates from one pooled sample per farm (n = 48) were tested for their phenotypical resistance against amoxicillin, tetracycline, cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole (TMP/SMX). Logistic regression analysis revealed the following risk factors (P < 0·05); farmer wearing the same work clothes for several days [ciprofloxacin, odds ratio (OR) 2·6; tetracycline, OR 2·4], administration of trimethoprim-sulfonamide combinations (TMP/SMX, OR 3·0; amoxicillin, OR 3·1; tetracycline, OR 2·6), ⩾0·3 animal daily dosage per production cycle (ADD/pc), quinolones (ciprofloxacin, OR 2·8), ⩾1·3 ADD/pc, penicillins (ciprofloxacin, OR 3·3; tetracycline, OR 3·4), 20-40 ADD/pc, tetracyclines (tetracycline, OR 3·2) and >40 ADD/pc, tetracyclines (tetracycline, OR 13·1; amoxicillin, OR 6·5). In this study antimicrobial resistance in commensal E. coli was mainly associated with antimicrobial drug use.
本研究的目的是确定包括抗菌药物使用在内的养殖场管理因素与来自白犊牛粪便的共生大肠杆菌分离株耐药性之间的关联。对每个养殖场（n = 48）一个混合样本中的90株大肠杆菌分离株进行了阿莫西林、四环素、头孢噻肟、环丙沙星和甲氧苄啶/磺胺甲恶唑（TMP/SMX）的表型耐药性检测。逻辑回归分析揭示了以下风险因素（P < 0·05）：农民连续几天穿同一工作服（环丙沙星，比值比[OR] 2·6；四环素，OR 2·4）、使用甲氧苄啶 - 磺胺类组合药物（TMP/SMX，OR 3·0；阿莫西林，OR 3·1；四环素，OR 2·6）、每个生产周期动物每日剂量⩾0·3（ADD/pc）、喹诺酮类药物（环丙沙星，OR 2·8）、ADD/pc⩾1·3、青霉素类药物（环丙沙星，OR 3·3；四环素，OR 3·4）、20 - 40 ADD/pc、四环素类药物（四环素，OR 3·2）以及>40 ADD/pc、四环素类药物（四环素，OR 13·1；阿莫西林，OR 6·5）。在本研究中，共生大肠杆菌的抗菌耐药性主要与抗菌药物的使用有关。
