Department of Respiratory Therapy, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
Pediatr Res. 2013 Nov;74(5):584-91. doi: 10.1038/pr.2013.142. Epub 2013 Sep 2.
Cysteinyl leukotrienes are important mediators of airway inflammation, whereas 8-isoprostane is a biomarker of oxidative stress. This study evaluated the distributions of cysteinyl leukotriene and 8-isoprostane concentrations in exhaled breath condensates (EBCs) of children. The relationship between cysteinyl leukotriene and 8-isoprostane concentrations in the EBCs was also evaluated.
The EBCs were collected from 34 children with allergic respiratory diseases and 24 healthy children. All recruited children underwent pulmonary function testing every season. The severity of allergic respiratory diseases and medication status were assessed every month in children with allergic respiratory diseases.
The EBC cysteinyl leukotriene and 8-isoprostane levels were higher in children with asthma and allergic rhinitis than in those with asthma only and healthy children. In asthmatic children, cysteinyl leukotriene and 8-isoprostane levels peaked in the summer. All children showed a clear association between EBC cysteinyl leukotriene and EBC 8-isoprostane levels.
The cysteinyl leukotriene and 8-isoprostane concentrations in the EBCs of children significantly varied by season. Oxidative stress correlated with airway inflammation in children.
半胱氨酰白三烯是气道炎症的重要介质，而 8-异前列腺素是氧化应激的生物标志物。本研究评估了儿童呼出冷凝物（EBC）中半胱氨酰白三烯和 8-异前列腺素浓度的分布。还评估了 EBC 中半胱氨酰白三烯和 8-异前列腺素浓度之间的关系。
从 34 名患有过敏性呼吸道疾病的儿童和 24 名健康儿童中收集 EBC。所有入组儿童每季度进行肺功能测试。在患有过敏性呼吸道疾病的儿童中，每月评估过敏性呼吸道疾病的严重程度和用药情况。
哮喘和过敏性鼻炎患儿的 EBC 半胱氨酰白三烯和 8-异前列腺素水平高于仅哮喘患儿和健康儿童。在哮喘儿童中，半胱氨酰白三烯和 8-异前列腺素水平在夏季达到峰值。所有儿童的 EBC 半胱氨酰白三烯和 EBC 8-异前列腺素水平之间均存在明显关联。
儿童 EBC 中的半胱氨酰白三烯和 8-异前列腺素浓度随季节显著变化。氧化应激与儿童气道炎症相关。
