1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58, Zhongshan Road II, Guangzhou 510080, China;
Int J Med Sci. 2013 Sep 24;10(12):1689-97. doi: 10.7150/ijms.7292. eCollection 2013.
DJ-1 and PTEN have been shown to involve in multiple cell processes and play an important role in cancer development and progression. However, their relationship with gastric carcinoma (GC) has not been identified yet. The purpose of this study is to clarify the relationship of DJ-1 and phosphatase and tensin homolog (PTEN) with clinicopathological parameters and prognosis in GC.
114 specimens were collected from GC patients and expression of DJ-1 and PTEN in tissue microarray was evaluated by immunohistochemical staining. Correlation between immunostainings and clinicopathological parameters, follow-up data of patients, was analyzed statistically.
High expression of DJ-1 was found in 66.7% (76/114) and associated with tumor depth (P=0.003), lymph node metastasis (P=0.011), distant metastasis (P=0.001) and advanced clinical stage (P=0.001). Loss or downregulation of PTEN was found in 58.7% (67/114) and associated with advanced clinical stage (P=0.018) and high expression of DJ-1 in tumor cells (P=0.006). In univariate survival analysis, high-expression of DJ-1 or loss of PTEN was significantly associated with poor prognosis of GC patients. However, only tumor depth (P=0.011) and coexistence of DJ-1 and PTEN abnormal expression (P=0.009) emerged as strong independent prognostic factors for overall survival of GC patients.
the present study indicates that DJ-1 and PTEN may play their roles in progression of GC in a cooperating pattern. Co-existence of abnormal DJ-1 and PTEN expression is likely to serve as an independent predictive factor for prognosis of GC patients.
DJ-1 和 PTEN 已被证明参与多种细胞过程，并在癌症的发生和发展中发挥重要作用。然而，它们与胃癌（GC）的关系尚未确定。本研究旨在阐明 DJ-1 和磷酸酶与张力蛋白同源物（PTEN）与 GC 临床病理参数和预后的关系。
收集 114 例 GC 患者的标本，采用免疫组织化学染色法检测组织微阵列中 DJ-1 和 PTEN 的表达。统计分析免疫染色与临床病理参数、患者随访资料的相关性。
发现 DJ-1 高表达率为 66.7%（76/114），与肿瘤深度（P=0.003）、淋巴结转移（P=0.011）、远处转移（P=0.001）和临床晚期（P=0.001）有关。发现 58.7%（67/114）的 PTEN 缺失或下调，与临床晚期（P=0.018）和肿瘤细胞中 DJ-1 高表达有关（P=0.006）。单因素生存分析显示，DJ-1 高表达或 PTEN 缺失与 GC 患者预后不良显著相关。然而，只有肿瘤深度（P=0.011）和 DJ-1 和 PTEN 异常表达共存（P=0.009）是 GC 患者总生存的独立预后因素。
本研究表明，DJ-1 和 PTEN 可能以协同模式在 GC 进展中发挥作用。DJ-1 和 PTEN 异常表达共存可能是 GC 患者预后的独立预测因子。
