State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.
Antonie Van Leeuwenhoek. 2014 Jan;105(1):99-107. doi: 10.1007/s10482-013-0057-4. Epub 2013 Oct 25.
A taxonomic study was carried out on strain HYC-10(T), which was isolated from the intestinal tract contents of a flathead mullet, Mugil cephalus, captured from the sea off Xiamen Island, China. The bacterium was observed to be Gram positive, oxidase and catalase positive, rod shaped, and motile by subpolar flagella. The bacterium was found to grow at salinities of 0-12 % and at temperatures of 8-45 °C. The isolate was found to hydrolyze aesculin and gelatin, but was unable to reduce nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HYC-10(T) belongs to the genus Bacillus, with highest sequence similarity (99.3 %) to Bacillus aerophilus 28K(T), Bacillus stratosphericus 41KF2a(T) and Bacillus altitudinis DSM 21631(T), followed by Bacillus safensis DSM 19292(T) (99.5 %) and Bacillus pumilus DSM 27(T) (99.5 %), while the sequence similarities to others were all below 97.6 %. The genomic ANIm values between strain HYC-10(T) and three type strains (B. altitudinis DSM 21631(T), B. safensis DSM 19292(T) and B. pumilus DSM 27(T)) were determined to range from 89.11 to 91.53 %. The DNA-DNA hybridization estimate values between strain HYC-10(T) and the three type strains were from 36.60 to 44.00 %. The principal fatty acids identified were iso-C15:0 (39.1 %), anteiso-C15:0 (22.7 %), iso-C17:0 (13.1 %), C16:0 (6.1 %), anteiso-C17:0 (5.8 %) and iso-C16:0 (5.1 %). The G+C content of the chromosomal DNA was determined from the draft genome sequence to be 41.3 mol%. The respiratory quinone was determined to be MK-7 (100 %). Phosphatidylglycerol, diphosphatidylglycerol, aminoglycolipid, two glycolipids and two unknown phospholipids were found to be present. The combined genotypic and phenotypic data show that strain HYC-10(T) represents a novel species of the genus Bacillus, for which the name Bacillus xiamenensis sp. nov. is proposed, with the type strain HYC-10(T) (=CGMCC NO.1.12326(T) = LMG 27143(T) = MCCC 1A00008(T)).
一项针对菌株 HYC-10(T)的分类学研究，该菌株从中国厦门岛海域捕获的平头鲷肠道内容物中分离得到。观察到该细菌为革兰氏阳性，氧化酶和过氧化氢酶阳性，杆状，具有极性鞭毛，可运动。该菌在 0-12%的盐度和 8-45°C 的温度下生长。该分离物能够水解马栗树皮苷和明胶，但不能将硝酸盐还原为亚硝酸盐。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HYC-10(T)属于芽孢杆菌属，与 Aerophilus 28K(T)、Stratosphericus 41KF2a(T)和 Altitudinis DSM 21631(T)的序列相似性最高（99.3%），其次是 Safensis DSM 19292(T)（99.5%）和 Pumilus DSM 27(T)（99.5%），而与其他菌株的序列相似性均低于 97.6%。菌株 HYC-10(T)与三种模式菌株（DSM 21631(T)、DSM 19292(T)和 DSM 27(T)）之间的基因组 ANIm 值确定为 89.11%至 91.53%。菌株 HYC-10(T)与三种模式菌株之间的 DNA-DNA 杂交估计值为 36.60%至 44.00%。鉴定的主要脂肪酸为 iso-C15:0（39.1%）、anteiso-C15:0（22.7%）、iso-C17:0（13.1%）、C16:0（6.1%）、anteiso-C17:0（5.8%）和 iso-C16:0（5.1%）。从基因组草图序列确定染色体 DNA 的 G+C 含量为 41.3 mol%。呼吸醌被确定为 MK-7（100%）。发现存在磷脂酰甘油、双磷脂酰甘油、氨基糖脂、两种糖脂和两种未知磷脂。综合表型和基因型数据表明，菌株 HYC-10(T)代表芽孢杆菌属的一个新种，提议将其命名为厦门芽孢杆菌 sp. nov.，其模式菌株为 HYC-10(T)=CGMCC NO.1.12326(T)=LMG 27143(T)=MCCC 1A00008(T)。
