Li Xiaoyi, Wang Liping, Huang Hui, Lai Qiliang, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.
Antonie Van Leeuwenhoek. 2014 Dec;106(6):1223-9. doi: 10.1007/s10482-014-0292-3. Epub 2014 Sep 25.
A novel bacterial strain designated P3-1(T) was isolated from the intestinal tract contents of Pacific white shrimp (Penaeus vannamei) in Zhangpu, Fujian province, China. The isolate was found to be Gram-negative, long rod shaped, oxidase- and catalase- positive. Growth was observed at 1-7 % sea salt (w/v, optimum, 3 %), at pH 7.0-9.0 (optimum, pH 7.0) and at 10-37 °C (optimum, 28 °C). The isolate was capable of hydrolysing gelatin, casein, starch and DNA but unable to degrade Tween 20, 40, 80 and cellulose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P3-1(T) belongs to the genus Aquimarina, with highest sequence similarity to Aquimarina gracilis PSC32(T) (96.2 %), followed by Aquimarina intermedia KMM 6258(T) (96.1 %), Aquimarina spongiae A6(T) (95.9 %) and Aquimarina muelleri KMM 6020(T) (95.8 %). The principal cellular fatty acids were identified as iso-C15:0, iso-C17:0 3OH, C16:1 ω7c/ω6c, iso-C15:1 G, iso-C15:0 3OH, iso-C17:1 ω9c/C16:0 10-methyl and C16:0. The G+C content of the chromosomal DNA was determined to be 33.3 mol%. The respiratory quinone was determined to be MK-6 (100 %). The combined genotypic and phenotypic data show that strain P3-1(T) represents a novel species within the genus Aquimarina, for which the name Aquimarina penaei sp. nov. is proposed, with the type strain P3-1(T) (=MCCC 1A09871(T) = LMG 27943(T)).
从中国福建省漳浦县的凡纳滨对虾（南美白对虾）肠道内容物中分离出一株新的细菌菌株，命名为P3-1(T)。该分离株为革兰氏阴性，长杆状，氧化酶和过氧化氢酶呈阳性。在1-7%海盐（质量/体积，最适为3%）、pH 7.0-9.0（最适pH 7.0）和10-37 °C（最适28 °C）条件下可生长。该分离株能够水解明胶、酪蛋白、淀粉和DNA，但不能降解吐温20、40、80和纤维素。基于16S rRNA基因序列的系统发育分析表明，菌株P3-1(T)属于海栖菌属，与纤细海栖菌PSC32(T)的序列相似性最高（96.2%），其次是中间海栖菌KMM 6258(T)（96.1%）、海绵海栖菌A6(T)（95.9%）和米勒海栖菌KMM 6020(T)（95.8%）。主要细胞脂肪酸鉴定为异-C15:0、异-C17:0 3OH、C16:1 ω7c/ω6c、异-C15:1 G、异-C15:0 3OH、异-C17:1 ω9c/C16:0 10-甲基和C16:0。染色体DNA的G+C含量测定为33.3 mol%。呼吸醌测定为MK-6（100%）。综合基因型和表型数据表明，菌株P3-1(T)代表海栖菌属内的一个新物种，为此提出新名称凡纳滨海栖菌（Aquimarina penaei sp. nov.），模式菌株为P3-1(T)（=MCCC 1A09871(T) = LMG 27943(T)）。
