Shin Hyun-Ho, Cho Seung-Hak
Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Osong, Korea.
Osong Public Health Res Perspect. 2013 Apr;4(2):72-5. doi: 10.1016/j.phrp.2013.03.001.
This study aimed to characterize the prevalence of antibiotic resistance in Escherichia coli isolates from the fecal samples of fishery workers who work in fish farms and often use antibiotics for the feeding fishes.
Seventy-three E. coli strains isolated from the fecal samples of fishery workers and 180 isolates from a control group of restaurant workers were tested for antibiotic resistance by agar disk diffusion with 16 antimicrobial agents.
About 30% of isolates from each group showed antimicrobial resistance to ampicillin, and 60% of isolates from fishery workers and 41% from restaurant workers were resistant to tetracycline. The isolates showed higher resistance to cephalothin and cefoxitin than to other cephem antibiotics and to gentamicin than to other aminogycosides. Our data indicated that fecal E. coli isolates from fishery workers showed higher antibiotic resistance than those of non-fishery workers (restaurant workers), especially to cephalothin, tetracycline, and trimethoprim-sulfamethoxazole (p < 0.05). However, rates of multidrug resistance were similar among the fishery workers and restaurant workers.
Frequent use of antibiotics may cause increased antibiotic resistance in the human microbiome.
本研究旨在描述在养鱼场工作且经常使用抗生素喂鱼的渔业工人粪便样本中分离出的大肠杆菌的抗生素耐药性流行情况。
采用琼脂纸片扩散法，用16种抗菌药物对从渔业工人粪便样本中分离出的73株大肠杆菌菌株以及从餐厅工作人员对照组中分离出的180株菌株进行抗生素耐药性检测。
每组约30%的分离株对氨苄西林表现出抗菌耐药性，60%的渔业工人分离株和41%的餐厅工作人员分离株对四环素耐药。分离株对头孢噻吩和头孢西丁的耐药性高于其他头孢菌素类抗生素，对庆大霉素的耐药性高于其他氨基糖苷类抗生素。我们的数据表明，渔业工人粪便中的大肠杆菌分离株比非渔业工人（餐厅工作人员）的分离株表现出更高的抗生素耐药性，尤其是对头孢噻吩、四环素和甲氧苄啶 - 磺胺甲恶唑（p < 0.05）。然而，渔业工人和餐厅工作人员的多重耐药率相似。
频繁使用抗生素可能会导致人类微生物组中抗生素耐药性增加。
