Ouanes A, Kouais A, Marouen S, Sahnoun M, Jemli B, Gargouri S
Service de parasitologie-mycologie, hôpital militaire principal d'instruction de Tunis, 1008 Montfleury, Tunis, Tunisie.
J Mycol Med. 2013 Dec;23(4):237-41. doi: 10.1016/j.mycmed.2013.07.058. Epub 2013 Oct 23.
The incidence of invasive candidiasis has increased dramatically over the last decades due to a larger number of patients at risk. The diagnosis remains difficult as the clinical presentation is not specific and the biological diagnosis usually takes several days to become positive. We propose in this work through a prospective study to evaluate the contribution of a chromogenic medium CHROMagar(®) (Becton-Dickinson) in the mycological diagnosis of Candida. We selected 680 samples from patients hospitalized in the intensive care unit for epidemiological surveillance over a period of 11 weeks. We treated samples by culture on Sabouraud and on CHROMagar(®). The species identification was performed by chlamydosporulation test and carbohydrate assimilation tests. We found that the CHROMagar(®)Candida evaluated in our work was a valuable tool in the primary culture in differentiating the most frequently isolated yeast species and in better detection of mixed cultures.
在过去几十年中，由于高危患者数量增多，侵袭性念珠菌病的发病率急剧上升。由于临床表现不具特异性且生物学诊断通常需要数天才能呈阳性，因此诊断仍然困难。在这项工作中，我们通过一项前瞻性研究，评估显色培养基CHROMagar®（碧迪医疗）在念珠菌真菌学诊断中的作用。我们从重症监护病房住院患者中选取了680份样本，进行了为期11周的流行病学监测。我们将样本分别接种在沙氏培养基和CHROMagar®上进行培养。通过厚垣孢子形成试验和碳水化合物同化试验进行菌种鉴定。我们发现，在我们的研究中评估的CHROMagar®念珠菌培养基，在原代培养中是一种有价值的工具，可以区分最常分离出的酵母菌种，并能更好地检测混合培养物。
