Badr Christian E
Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.
Methods Mol Biol. 2014;1098:1-18. doi: 10.1007/978-1-62703-718-1_1.
Over the last three decades, imaging has been a thriving field with continuous egression of more reliable and highly sophisticated tools and techniques allowing better understanding of biological processes in living organisms. This field continues to expand and its applications broaden to encompass limitless applications in various biomedical research areas. It is however, of utmost importance to understand the capabilities and limitations of this technique as new challenges and hurdles continue to arise. This chapter describes the general properties of bioluminescence imaging and commonly used reporters while underlining the challenges and limitations with these modalities.
在过去三十年中，成像一直是一个蓬勃发展的领域，更可靠、高度复杂的工具和技术不断涌现，使人们能够更好地理解生物体中的生物过程。该领域持续扩展，其应用范围不断拓宽，涵盖了各种生物医学研究领域中无数的应用。然而，随着新的挑战和障碍不断出现，了解这项技术的能力和局限性至关重要。本章描述了生物发光成像的一般特性和常用的报告基因，同时强调了这些方法所面临的挑战和局限性。
