Isfahan Cardiovascular Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Dis Markers. 2013;35(4):273-80. doi: 10.1155/2013/612035. Epub 2013 Sep 12.
Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1). Fatty acid composition of HDL could affect its size and structure. On the other hand, PON1 activity is directly related to the structure of HDL. This study was designed to investigate the association between serum PON1 activity and fatty acid composition of HDL in healthy men.
One hundred and forty healthy men participated in this research. HDL was separated by sequential ultracentrifugation, and its fatty acid composition was analyzed by gas chromatography. PON1 activity was measured spectrophotometrically using paraxon as substrate.
Serum PON1 activity was directly correlated with the amount of stearic acid and dihomo-gamma-linolenic acid (DGLA). PON1/HDL-C was directly correlated with the amount of miristic acid, stearic acid, and DGLA and was inversely correlated with total amount of ω 6 fatty acids of HDL.
The fatty acid composition of HDL could affect the activity of its associated enzyme, PON1. As dietary fats are the major determinants of serum lipids and lipoprotein composition, consuming some special dietary fatty acids may improve the activity of PON1 and thereby have beneficial effects on health.
高密度脂蛋白（HDL）的心脏保护作用部分依赖于与其相关的酶——对氧磷酶 1（PON1）。HDL 的脂肪酸组成可以影响其大小和结构。另一方面，PON1 活性直接与 HDL 的结构相关。本研究旨在调查健康男性血清 PON1 活性与 HDL 脂肪酸组成之间的关系。
140 名健康男性参与了这项研究。采用连续超速离心法分离 HDL，并通过气相色谱法分析其脂肪酸组成。使用对氧磷作为底物，分光光度法测定 PON1 活性。
血清 PON1 活性与硬脂酸和二高-γ-亚麻酸（DGLA）的含量直接相关。PON1/HDL-C 与肉豆蔻酸、硬脂酸和 DGLA 的含量呈正相关，与 HDL 中 ω6 脂肪酸的总量呈负相关。
HDL 的脂肪酸组成可以影响与其相关的酶 PON1 的活性。由于膳食脂肪是血清脂质和脂蛋白组成的主要决定因素，因此摄入某些特殊的膳食脂肪酸可能会提高 PON1 的活性，从而对健康产生有益的影响。
