Hiesel R, von Haeseler A, Brennicke A
Institute für Genbiologische Forschung, Berlin, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):634-8. doi: 10.1073/pnas.91.2.634.
To evaluate the potential of mitochondrial nucleic acid sequences as a phylogenetic tool, we have analyzed cytochrome oxidase subunit III (coxIII) coding sequences in representatives of the major groups of land plants. The phylogenetic tree derived from these mitochondrial sequences confirms the monophyletic origin of land plant mitochondria with the general order and descent of land plants deduced by other molecular, physiological, and morphological traits. The mitochondrial sequences strongly suggest a close phylogenetic relationship between Bryophyta and Lycopodiatae, whereas Psilophytatae cluster with the other vascular plants. In addition to the high sequence similarity, both Hepaticophytina and Lycopodiatae contain a related intron in the coxIII gene that, to our knowledge, is not found in any other plant species. The slowly evolving mitochondrial sequences of plants are shown to provide a useful phylogenetic tool to evaluate distant evolutionary relationships within this kingdom.
为了评估线粒体核酸序列作为一种系统发育工具的潜力,我们分析了主要陆地植物类群代表中的细胞色素氧化酶亚基III(coxIII)编码序列。从这些线粒体序列推导出来的系统发育树证实了陆地植物线粒体的单系起源,以及通过其他分子、生理和形态特征推断出的陆地植物的一般顺序和演化关系。线粒体序列有力地表明苔藓植物和石松纲之间存在密切的系统发育关系,而裸蕨纲则与其他维管植物聚在一起。除了高度的序列相似性外,苔纲和石松纲在coxIII基因中都含有一个相关的内含子,据我们所知,在任何其他植物物种中都未发现。研究表明,植物缓慢进化的线粒体序列为评估该植物界内遥远的进化关系提供了一种有用的系统发育工具。