Ujino-Ihara T, Yoshimura K, Ugawa Y, Yoshimaru H, Nagasaka K, Tsumura Y
Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki, Japan.
Plant Mol Biol. 2000 Jul;43(4):451-7. doi: 10.1023/a:1006492103063.
To assist genetic research into Cryptomeria japonica, which is one of the most important forest tree species in Japan, expressed sequence tag (EST) analysis was carried out. The cDNA clones were isolated from a library derived from inner bark tissues. Partial sequences were obtained from 2231 clones, representing 1398 unique transcripts. Putative functions were assigned to 1583 clones, which represented 882 unique transcripts, by a Blast algorithm. Homology analysis suggested that ESTs related to cell wall formation represented about 3% of the clones. Transcripts of plant stress response genes were also abundant in the inner bark library, especially genes involved in wounding and drought responses. This indicates that the stress response systems of this tree species are similar to those of other plants, and that these systems are highly conserved among plant species. The remaining 648 clones, which represented 516 unique transcripts, did not show any significant homology to known sequences in the databases searched: these are expected to represent genes specific to Cryptomeria and, possibly, to related species.
为辅助对日本最重要的林木树种之一日本柳杉的基因研究，开展了表达序列标签（EST）分析。从来源于内皮组织的文库中分离出cDNA克隆。从2231个克隆中获得了部分序列，代表1398个独特转录本。通过Blast算法为1583个克隆（代表882个独特转录本）赋予了推定功能。同源性分析表明，与细胞壁形成相关的EST约占克隆的3%。植物应激反应基因的转录本在内皮文库中也很丰富，尤其是参与伤口和干旱反应的基因。这表明该树种的应激反应系统与其他植物相似，并且这些系统在植物物种中高度保守。其余648个克隆（代表516个独特转录本）在搜索的数据库中与已知序列没有任何显著同源性：预计这些代表日本柳杉特有的基因，可能还有相关物种特有的基因。
