Ramaekers F, Feitz W, Moesker O, Schaart G, Herman C, Debruyne F, Vooijs P
Virchows Arch A Pathol Anat Histopathol. 1985;408(2-3):127-42. doi: 10.1007/BF00707977.
Thirteen primary and metastatic testicular germ cell tumours, including classical and anaplastic seminomas, and non-seminomatous testicular tumours were examined for their intermediate filament protein (IFP) types. The seminomas were shown to react with a monoclonal and a polyclonal antibody to bovine lens vimentin, while non-seminomatous germ cell tumours were strongly positive for a polyclonal and a monoclonal antibody to cytokeratin. In one case of seminoma with elevated serum levels of beta HCG and alpha FP, cytokeratin positive tumour cells were found. In the case of teratocarcinoma, several components of the tumour could be distinguished using a combination of antisera in double-label immunofluorescence microscopy. The glandular component of this tumour was positive with the polyclonal antikeratin, but also with the monoclonal cytokeratin antibody specific for glandular epithelia (RGE 53). However, the squamous component was negative with this latter antibody. Strikingly, the spindle cell component showed focal positivity for vimentin, with coexpression of cytokeratin and vimentin in some cells. Our data show that antibodies to cytokeratin and vimentin can be helpful in the diagnosis of testicular germ cell tumours, especially in the differentiation between seminomas and non-seminomatous tumours.
对13例原发性和转移性睾丸生殖细胞肿瘤进行了检查，包括经典型和间变型精原细胞瘤以及非精原细胞瘤性睾丸肿瘤，以确定它们的中间丝蛋白（IFP）类型。精原细胞瘤与抗牛晶状体波形蛋白的单克隆抗体和多克隆抗体发生反应，而非精原细胞瘤性生殖细胞肿瘤对抗细胞角蛋白的多克隆抗体和单克隆抗体呈强阳性。在1例血清β-HCG和α-FP水平升高的精原细胞瘤病例中，发现了细胞角蛋白阳性的肿瘤细胞。在畸胎癌病例中，使用双标记免疫荧光显微镜中的抗血清组合可以区分肿瘤的几个成分。该肿瘤的腺性成分对多克隆抗角蛋白呈阳性，但对腺上皮特异性的单克隆细胞角蛋白抗体（RGE 53）也呈阳性。然而，鳞状成分对后一种抗体呈阴性。引人注目的是，梭形细胞成分对波形蛋白呈局灶性阳性，在一些细胞中细胞角蛋白和波形蛋白共表达。我们的数据表明，抗细胞角蛋白和波形蛋白的抗体有助于睾丸生殖细胞肿瘤的诊断，特别是在精原细胞瘤和非精原细胞瘤之间的鉴别诊断中。
