用IgE抗体对小鼠和大鼠进行被动致敏。

Passive sensitization of mice and rats with IgE antibodies.

作者信息

Wyczółkowska J, Marciniak T, Brzezińska-Błaszczyk E, Maśliński C

出版信息

Arch Immunol Ther Exp (Warsz). 1978;26(1-6):689-93.

Abstract

The strain-dependent variation in susceptibility to passive sensitization of the skin and peritoneal mast cells with homologous IgE antibodies was observed in inbred strains of mice. Strain 129 showed low susceptibility to passive sensitization and high efficiency of normal serum in blocking mouse reagin-induced PCA in rats. Balb/c and C3H/A strains showed higher susceptibility to passive sensitization and the efficiency of normal serum in blocking rat PCA lower than strain 129. The serum factor responsible for rat PCA inhibiting effect was heat-labile. The anaphylactic response of peritoneal mast cells of inbred strains to the challenge with anti-mouse IgE and anti-rat IgE in vitro was low in Balb/c mice, as compared with the response of C57BL/6J and 129 mice. The results suggest, that non-specific IgE present in the serum or bound to the mast cells may be one of the factors determining the susceptibility of mouse strains to reagin-induced passive sensitization. However, the results obtained with C57BL/6J strain suggest the existence of other factors effecting this susceptibility.

摘要

在近交系小鼠中观察到，皮肤和腹膜肥大细胞对同源IgE抗体被动致敏的易感性存在品系依赖性差异。129品系对被动致敏的易感性较低，其正常血清在阻断大鼠中鼠反应素诱导的被动皮肤过敏反应（PCA）方面效率较高。Balb/c和C3H/A品系对被动致敏的易感性较高，其正常血清阻断大鼠PCA的效率低于129品系。负责大鼠PCA抑制作用的血清因子对热不稳定。与C57BL/6J和129小鼠相比，Balb/c小鼠近交系腹膜肥大细胞在体外对抗小鼠IgE和抗大鼠IgE攻击的过敏反应较低。结果表明，血清中存在的或与肥大细胞结合的非特异性IgE可能是决定小鼠品系对反应素诱导的被动致敏易感性的因素之一。然而，C57BL/6J品系获得的结果表明存在影响这种易感性的其他因素。