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通过等电聚焦对牛奶中的遗传变异进行同步表型分析的方法

Procedure for simultaneous phenotyping of genetic variants in cow's milk by isoelectric focusing.

作者信息

Seibert B, Erhardt G, Senft B

出版信息

Anim Blood Groups Biochem Genet. 1985;16(3):183-91. doi: 10.1111/j.1365-2052.1985.tb01469.x.

DOI:10.1111/j.1365-2052.1985.tb01469.x
PMID:4073592
Abstract

A method is described which allows the simultaneous separation of all polymorphic protein fractions in cow's milk in one single run. The separation could be achieved by isoelectric focusing in ultrathin-layer polyacrylamide gels. The method is especially suitable for screening purposes because it combines low costs, high resolution and short separation time.

摘要

本文描述了一种方法,该方法能够在一次操作中同时分离牛奶中的所有多态性蛋白质组分。这种分离可以通过在超薄层聚丙烯酰胺凝胶中进行等电聚焦来实现。该方法特别适用于筛选目的,因为它兼具低成本、高分辨率和短分离时间的特点。

相似文献

1
Procedure for simultaneous phenotyping of genetic variants in cow's milk by isoelectric focusing.通过等电聚焦对牛奶中的遗传变异进行同步表型分析的方法
Anim Blood Groups Biochem Genet. 1985;16(3):183-91. doi: 10.1111/j.1365-2052.1985.tb01469.x.
2
Phenotyping of bovine milk proteins by reversed-phase high-performance liquid chromatography.
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Transferrin variants in sheep: separation and characterization by polyacrylamide gel electrophoresis and isoelectric focusing.绵羊中的转铁蛋白变体:通过聚丙烯酰胺凝胶电泳和等电聚焦进行分离与表征
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Determination of cow's milk in non-bovine and mixed cheeses by capillary electrophoresis of whey proteins in acidic isoelectric buffers.在酸性等电缓冲液中通过乳清蛋白的毛细管电泳测定非牛奶奶酪和混合奶酪中的牛奶成分。
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[Quantitative differences between genetic variants of milk proteins in Cholmogor cattle].
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引用本文的文献

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Does a Little Difference Make a Big Difference? Bovine β-Casein A1 and A2 Variants and Human Health-An Update.差异虽小,影响却大?牛 β-酪蛋白 A1 和 A2 变体与人类健康——最新进展。
Int J Mol Sci. 2022 Dec 9;23(24):15637. doi: 10.3390/ijms232415637.
2
Genetic diversity and population structure in divergent German cattle selection lines on the basis of milk protein polymorphisms.基于乳蛋白多态性的德国不同奶牛选育品系的遗传多样性和群体结构
Arch Anim Breed. 2021 Mar 11;64(1):91-102. doi: 10.5194/aab-64-91-2021. eCollection 2021.
3
Comparative Effects of Milk Containing A1 versus A2 β-Casein on Health, Growth and β-Casomorphin-7 Level in Plasma of Neonatal Dairy Calves.
含A1与A2 β-酪蛋白的牛奶对新生奶牛犊健康、生长及血浆中β-酪蛋白吗啡-7水平的比较影响
Animals (Basel). 2020 Dec 30;11(1):55. doi: 10.3390/ani11010055.
4
Associations between maternal milk protein genotypes with preweaning calf growth traits in beef cattle.牛母乳蛋白基因型与犊牛生长性状的关系。
J Anim Sci. 2020 Oct 1;98(10). doi: 10.1093/jas/skaa280.
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Variants within the 5'-flanking regions of bovine milk protein genes: I. κ-casein-encoding gene.牛乳蛋白基因 5'侧翼区的变异:I. κ-酪蛋白编码基因。
Theor Appl Genet. 1994 Sep;89(1):116-20. doi: 10.1007/BF00226992.
6
Associations between 2 paternal casein haplotypes and milk yield traits of Swiss Fleckvieh cattle.瑞士弗莱维赫牛的两种父本酪蛋白单倍型与产奶性状之间的关联。
J Appl Genet. 2008;49(1):69-74. doi: 10.1007/BF03195250.
7
Ultrathin-layer isoelectric focusing for bovine serum transferrin phenotyping.用于牛血清转铁蛋白表型分析的超薄层等电聚焦法。
Biochem Genet. 1989 Aug;27(7-8):425-9. doi: 10.1007/BF02399671.