Seibert B, Erhardt G, Senft B
Anim Blood Groups Biochem Genet. 1985;16(3):183-91. doi: 10.1111/j.1365-2052.1985.tb01469.x.
A method is described which allows the simultaneous separation of all polymorphic protein fractions in cow's milk in one single run. The separation could be achieved by isoelectric focusing in ultrathin-layer polyacrylamide gels. The method is especially suitable for screening purposes because it combines low costs, high resolution and short separation time.
本文描述了一种方法,该方法能够在一次操作中同时分离牛奶中的所有多态性蛋白质组分。这种分离可以通过在超薄层聚丙烯酰胺凝胶中进行等电聚焦来实现。该方法特别适用于筛选目的,因为它兼具低成本、高分辨率和短分离时间的特点。