Pflanzenphysiologisches Institut und Botanischer Garten der Universität, Untere Karspüle 2, W-3400, Göttingen, Federal Republic of Germany.
Planta. 1992 Sep;188(2):252-7. doi: 10.1007/BF00216821.
Gibberellin (GA) metabolism from GA12-aldehyde was studied in cell-free systems from 2-d-old germinating embryos of barley. [(14)C]- or [17-(2)H2]Gibberellins were used as substrates and all products were identified by combined gas chromatography-mass spectrometry. Stepwise analysis demonstrated the conversion of GA12-aldehyde via the 13-deoxy pathway to GA51 and via the 13-hydroxylation pathway to GA29, GA1 and GA8. In addition, GA3 was formed from GA20 via GA5. We conclude that the embryo is capable of producing gibberellins that can induce α-amylase production in the aleurone layer. There was no evidence for 12β- or 18-hydroxylation and GA4 was neither synthesised nor metabolised by the system. All metabolically obtained GAs, with the exception of GA3, were also found as endogenous components of the cell-free system in spite of ammonium-sulfate precipitation and desalting steps.
我们以萌发 2 天的大麦幼胚的无细胞体系为材料，研究了从 GA12-醛到赤霉素（GA）的代谢。我们使用了 [(14)C]-或 [17-(2)H2]赤霉素作为底物，并用气相色谱-质谱联用技术对所有产物进行了鉴定。逐步分析表明，GA12-醛通过 13-脱氧途径转化为 GA51，通过 13-羟基化途径转化为 GA29、GA1 和 GA8。此外，GA3 可由 GA20 通过 GA5 形成。我们的结论是，胚胎能够产生赤霉素，从而诱导糊粉层中α-淀粉酶的产生。没有证据表明存在 12β-或 18-羟化，该体系也没有合成或代谢 GA4。尽管经过硫酸铵沉淀和脱盐步骤，所有代谢获得的 GA（除了 GA3）也被发现是无细胞体系的内源性成分。
