Damian E, Ianăş O, Bădescu I
Endocrinologie. 1985 Oct-Dec;23(4):247-51.
A melatonin-free pineal extract administered to rats in doses of 2 ml/day/animal along 12 days, induced a statistically significant drop in urinary 17 KS, cholesterolemia, hepatic, adrenal and testicular cholesterol. The dose of 1 microgram melatonin/day/animal did not alter these biochemical indices. This dose administered for 3 days did not affect the serum level and testicular concentration of testosterone, unlike the pineal extract which lowered significantly serum and testicular testosterone. Unlike the pineal extract administration of melatonin in doses of 10 micrograms/day/animal along 3 days had no effect on DNA, RNA and testicular proteins. Administration of the pineal extract lowered significantly serum LH and FSH after 3 days whereas melatonin in doses of 50 micrograms/day/animal did not have a similar effect. Furthermore, while the pineal extract inhibited the post-castration elevation of LH and FSH, melatonin in doses of 50 micrograms/day/animal had no effect. The same difference was noted with rats under GnRH stimulation: the pineal extract inhibited the pituitary response to GnRH whereas melatonin did not. The authors conclude that the effects produced by the pineal extract are not mediated by melatonin.
以每天每只动物2毫升的剂量给大鼠连续12天注射不含褪黑素的松果体提取物，可使尿17酮类固醇、胆固醇血症、肝脏、肾上腺和睾丸胆固醇含量在统计学上显著下降。每天每只动物1微克褪黑素的剂量并未改变这些生化指标。连续3天给予该剂量对睾酮的血清水平和睾丸浓度没有影响，而松果体提取物则会显著降低血清和睾丸中的睾酮水平。与松果体提取物不同，每天每只动物10微克的褪黑素连续3天给药对DNA、RNA和睾丸蛋白没有影响。给药3天后，松果体提取物可显著降低血清促黄体生成素（LH）和促卵泡生成素（FSH），而每天每只动物50微克的褪黑素则没有类似作用。此外，虽然松果体提取物可抑制去势后LH和FSH的升高，但每天每只动物50微克的褪黑素却没有此作用。在促性腺激素释放激素（GnRH）刺激下的大鼠中也观察到了同样的差异：松果体提取物可抑制垂体对GnRH的反应，而褪黑素则无此作用。作者得出结论，松果体提取物产生的作用并非由褪黑素介导。
