水痘带状疱疹病毒（VZV）的多肽以及VZV与单纯疱疹病毒（HSV）的免疫关系。

Polypeptides of varicella-zoster virus (VZV) and immunological relationship of VZV and herpes simplex virus (HSV).

作者信息

Shiraki K, Okuno T, Yamanishi K, Takahashi M

出版信息

J Gen Virol. 1982 Aug;61 (Pt 2):255-69. doi: 10.1099/0022-1317-61-2-255.

Abstract

Varicella-zoster virus (VZV), labelled with [35S]methionine or [14C]glucosamine, was purified by centrifugation through sucrose gradients followed by equilibrium centrifugation in CsCl, and analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). At least 32 polypeptides ranging in mol. wt. from approx. 280 X 10(3) to 21.5 X 10(3) (280K to 21.5K) and six glycopeptides ranging in mol. wt. from approx. 115K to 45K (gp1 to gp6) were found in the virion. The immunological relationship of VZV and herpes simplex virus (HSV) was investigated. In neutralization (NT) tests, no cross-neutralization was observed between VZV and HSV-1 or -2. In fluorescent antibody staining, however, a cross-reaction was observed between VZV- and HSV-1-infected human embryonic lung (HEL) cells and heterologous antiserum. When cross-reactions were investigated by immunoprecipitation followed by SDS-PAGE, several cross-reacting polypeptides were discovered. Cross-reacting glycopeptides of 64K (gp3) and 55K (gp5) were isolated from VZV-infected cell lysates by affinity column chromatography to immobilized HSV-1 antibodies.

摘要

用[35S]甲硫氨酸或[14C]葡糖胺标记的水痘带状疱疹病毒（VZV），先通过蔗糖梯度离心，再经CsCl平衡离心进行纯化，然后通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分析。在病毒粒子中发现了至少32种分子量范围约为280×10(3)至21.5×10(3)（280K至21.5K）的多肽以及6种分子量范围约为115K至45K（gp1至gp6）的糖肽。对VZV和单纯疱疹病毒（HSV）的免疫关系进行了研究。在中和（NT）试验中，未观察到VZV与HSV-1或-2之间的交叉中和现象。然而，在荧光抗体染色中，观察到VZV感染和HSV-1感染的人胚肺（HEL）细胞与异种抗血清之间存在交叉反应。当通过免疫沉淀继以SDS-PAGE研究交叉反应时，发现了几种交叉反应多肽。通过固定化HSV-1抗体的亲和柱色谱法，从VZV感染的细胞裂解物中分离出了64K（gp3）和55K（gp5）的交叉反应糖肽。