School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
Oncol Rep. 2014 Jan;31(1):450-5. doi: 10.3892/or.2013.2835. Epub 2013 Nov 5.
Ron is a human receptor for the macrophage-stimulating protein (MSP). Exon 11 skipping of Ron pre-mRNA produces the Ron∆165 protein that has a deletion of a 49 amino acid region in the β-chain extracellular domain. Ron∆165 is constitutively active even in the absence of its ligand. Through stepwise deletion analysis, we identified a 2-nt RNA enhancer, which is located 74 nt upstream from the 5' splice site of exon 11, for exon 11 inclusion. Through double-base and single-base substitution analysis of the 2-nt RNA, we demonstrated that the GA, CC, UG and AC dinucleotides on exon 11, in addition to the wild-type AG sequence, function as enhancers for exon 11 inclusion of the Ron pre-mRNA.
罗恩是巨噬细胞刺激蛋白 (MSP) 的人体受体。Ron 前 mRNA 的外显子 11 跳跃产生 Ron∆165 蛋白，该蛋白在β-链细胞外结构域缺失了 49 个氨基酸。即使没有配体，Ron∆165 也是组成性激活的。通过逐步缺失分析，我们鉴定了一个 2-nt RNA 增强子，它位于外显子 11 的 5'剪接位点上游 74nt，用于外显子 11 的包含。通过对 2-nt RNA 的双碱基和单碱基替换分析，我们证明了外显子 11 上的 GA、CC、UG 和 AC 二核苷酸，除了野生型 AG 序列外，还可以作为 Ron 前 mRNA 外显子 11 包含的增强子。
