Department of Microbial Biotechnology, Horticulture Research International, BN17 6LP, Littlehampton, West Sussex, UK.
Microb Ecol. 1993 Nov;26(3):277-85. doi: 10.1007/BF00176959.
The effect of lettuce (Latuca sativa L.) germination and growth in nonsterilized potting compost of 0.1% and 1.0% w/w incorporation of fermenter biomass inocula of six strains of Trichoderma was investigated. Except for strains WT and T35 at 0.1 % w/w, all inocula inhibited germination. Biomass of strains WT, T35, 20, and 47 at 1.0% promoted shoot fresh weight, whereas strains TH1 and 8MF2 were inhibitory. In contrast, when biomass of strains WT, TH1, and 8MF2 was autoclaved and incorporated at 1%, shoot fresh weight was promoted, but the biomass of T35 was inhibitory. None of the strains incorporated at 0.1 % w/w increased shoot fresh weight, and autoclaved biomass of TH1, T35, and 20 incorporated at 0.1% w/w resulted in lower shoot fresh weights in comparison with uninoculated controls. The shoot dry weight of lettuce seedlings could be enhanced by germinating seeds in uninoculated compost and after five days' growth transferring them into WT-inoculated compost. Inoculum of strain TH1 when applied using this method was very inhibitory. With WT the degree of increase in shoot fresh weight and germination rate declined as the fermentation time to produce inocula was increased.
研究了在未灭菌的盆栽堆肥中加入 0.1%和 1.0%w/w 的发酵罐生物量接种物（六种木霉菌株）对莴苣（Latuca sativa L.）发芽和生长的影响。除了 0.1%w/w 下的 WT 和 T35 菌株外，所有接种物均抑制发芽。WT、T35、20 和 47 菌株在 1.0%w/w 下促进了鲜重，而 TH1 和 8MF2 菌株则具有抑制作用。相比之下，当 WT、TH1 和 8MF2 菌株的生物量在 1%下进行高压灭菌并加入时，鲜重增加，但 T35 的生物量则具有抑制作用。在 0.1%w/w 下加入任何一种菌株均未增加鲜重，而 TH1、T35 和 20 的生物量在 0.1%w/w 下进行高压灭菌后，与未接种对照相比，鲜重降低。在未接种的堆肥中发芽种子，并在生长五天后将其转移到 WT 接种的堆肥中，可以提高生菜幼苗的地上部鲜重。采用这种方法时，TH1 菌株的接种物具有很强的抑制作用。随着接种物发酵时间的增加，WT 菌株地上部鲜重和发芽率的增加程度均下降。
