Shakib F, Brown H M, Phelps A, Redhead R
Int Arch Allergy Appl Immunol. 1986;79(4):349-56. doi: 10.1159/000234001.
ELISA plates coated with highly pure IgG4 were employed to detect IgM antiglobulin in atopic sera. The use of IgG4, rather than whole IgG, on the solid-phase was to provide direct evidence for the IgG4 reactivity of the antiglobulin. Bound IgM was shown to be antiglobulin in that binding can be inhibited by pre-absorption of serum with IgG. Some 75% of asthmatic patients and 29% of eczema patients were found to have significantly raised level of IgM antiglobulin. This antiglobulin resembles rheumatoid factor in that it appears to be directed against antigenic determinants common to human and rabbit IgG. Isolated antiglobulin-enriched IgM fractions released histamine from leucocytes of 7 out of 12 atopic patients. The histamine-releasing capacity of the IgM antiglobulin was shown to be operating via basophil-bound IgG in that the process can be blocked by pre-absorption of the antiglobulin with IgG. Furthermore, heating the antiglobulin-enriched IgM fraction did not affect its histamine-releasing capacity. We conclude that the IgM antiglobulin detectable in our atopic patients may contribute to the pathological changes in them.
用包被有高纯度IgG4的酶联免疫吸附测定（ELISA）板检测特应性血清中的IgM抗球蛋白。在固相上使用IgG4而非完整的IgG，是为了提供抗球蛋白IgG4反应性的直接证据。结合的IgM被证明是抗球蛋白，因为血清与IgG预吸收可抑制其结合。约75%的哮喘患者和29%的湿疹患者被发现IgM抗球蛋白水平显著升高。这种抗球蛋白类似于类风湿因子，因为它似乎针对人及兔IgG共有的抗原决定簇。从12名特应性患者中的7名患者的白细胞中，分离出的富含抗球蛋白的IgM组分可释放组胺。IgM抗球蛋白的组胺释放能力被证明是通过嗜碱性粒细胞结合的IgG起作用，因为该过程可被抗球蛋白与IgG预吸收所阻断。此外，加热富含抗球蛋白的IgM组分并不影响其组胺释放能力。我们得出结论，在我们的特应性患者中可检测到的IgM抗球蛋白可能促成了他们的病理变化。
