Plant Breeding Laboratory, Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan.
Theor Appl Genet. 1993 Mar;86(1):1-5. doi: 10.1007/BF00223802.
Protoplasts isolated from leaf mesophyll cells of Dianthus chinensis and D. barbatus were fused by polyethylene glycol (PEG). Calli exhibiting vigorous growth were selected from the PEG-treated protoplasts and shoots were regenerated from one of these calli after 5 months of culture. These shoots readily rooted and continuously produced flowers in the in-vitro condition. The data on flower color, chromosome number, and esterase isozyme patterns indicated that this plantlet was an interspecific somatic hybrid. The hybridity of the plantlet was also confirmed by nuclear rDNA analysis. This report provides the possibility of applying the somatic hybridization technique for the genetic improvement of the genus Dianthus.
从中国石竹和沟叶石竹的叶片叶肉细胞中分离出的原生质体通过聚乙二醇（PEG）融合。从经 PEG 处理的原生质体中选择出表现出旺盛生长的愈伤组织，并在 5 个月的培养后从其中一个愈伤组织中再生出芽。这些芽很容易生根，并在体外条件下持续开花。花色、染色体数目和酯酶同工酶模式等数据表明，该植株是一种种间体细胞杂种。核 rDNA 分析也证实了该植株的杂种特性。本报告为应用体细胞杂交技术进行石竹属遗传改良提供了可能。
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