Plant Breeding Laboratory, Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan.
Plant Cell Rep. 1992 Jun;11(5-6):225-8. doi: 10.1007/BF00235070.
Seventeen cultivars belonging to the genus Dianthus were examined for protoplast isolation, culture and shoot regeneration under the same conditions. These included D. caryophyllus, D. chinensis, D. barbatus, D. plumarius, D. superbus and D. japonicus as well as interspecific hybrid cultivars (D. caryophyllus x D. chinensis and D. chinensis x D. barbatus). In all cultivars, viable protoplasts were isolated at high yields from leaves of axenic shoot cultures and some of these protoplasts divided and formed colonies. However, shoot regeneration frequencies were markedly different among the species. High frequency shoot regeneration was obtained from D. chinensis and interspecific hybrid cultivars, while only low frequency or no shoot regeneration was obtained from other species.
对 17 个石竹属品种进行了原生质体分离、培养和芽再生的研究，这些品种包括 D. caryophyllus、D. chinensis、D. barbatus、D. plumarius、D. superbus 和 D. japonicus 以及种间杂交品种（D. caryophyllus x D. chinensis 和 D. chinensis x D. barbatus）。在所有品种中，均能从无菌芽培养的叶片中获得高产的有活力原生质体，其中一些原生质体分裂并形成菌落。然而，不同物种的芽再生频率差异显著。D. chinensis 和种间杂交品种的芽再生频率较高，而其他物种的芽再生频率较低或没有。
