电融合获得的石竹属和满天星属间属间体细胞杂种苗。

Intergeneric somatic hybrid plantlets between Dianthus barbatus and Gypsophila paniculata obtained by electrofusion.

机构信息

Graduate School of Science and Technology, Niigata University, 2-8050 Ikarashi, 950-21, Niigata, Japan.

出版信息

Theor Appl Genet. 1996 Feb;92(2):170-2. doi: 10.1007/BF00223372.

Abstract

Hypocotyl-derived protoplasts of Dianthus barbatus that had been pretreated with iodoacetamide were fused electrically with cell suspension culture-derived protoplasts of Gypsophila paniculata that could divide to form callus but could not regenerate shoots under the culture conditions used in this study. Electrofusion-derived calli which produced shoots were selected as putative somatic hybrids, and plantlets were subsequently regenerated from 2 of these selected calli. These plantlets, which in vitro produced flowers precociously, were identified as intergeneric somatic hybrids by nuclear ribosomal DNA analysis. Normal plants have not been established up to the present.

摘要

预处理碘乙酰胺的石竹下胚轴原生质体与在本研究中所用培养条件下能分裂形成愈伤组织但不能再生芽的满天星悬浮细胞原生质体通过电融合。选择能产生芽的电融合愈伤组织作为可能的体细胞杂种，随后从这 2 个选择的愈伤组织中再生出植株。这些在体外提前开花的植株通过核核糖体 DNA 分析被鉴定为属间体细胞杂种。到目前为止，还没有建立正常的植株。