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农杆菌与鹰嘴豆相互作用中的菌株和品种特异性。

Strain and cultivar specificity in the Agrobacterium-chickpea interaction.

机构信息

Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh.

出版信息

Plant Cell Rep. 1994 Jul;13(10):561-3. doi: 10.1007/BF00234511.

DOI:10.1007/BF00234511
PMID:24196221
Abstract

The susceptibility of four genotypes of chickpea to four wild strains of Agrobacterium tumefaciens was evaluated. Successful transformation was dependent on specific bacterial strain-plant cultivar interactions. Agropine strain A281 was the most effective for tumor induction. Tumors displayed hormone autonomous growth, were opine positive and contained DNA that was homologous to the T-DNA of the inciting strain.

摘要

评估了四个基因型的鹰嘴豆对四种野生型根癌农杆菌的敏感性。成功的转化取决于特定的细菌菌株-植物品种相互作用。农杆菌 A281 菌株是诱导肿瘤形成最有效的菌株。肿瘤表现出激素自主生长,呈(opine)阳性,并含有与激发菌株的 T-DNA 同源的 DNA。

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本文引用的文献

1
Gene transfer into peanut (Arachis hypogaea L.) by Agrobacterium tumefaciens.利用根癌农杆菌将基因转入花生(落花生)中。
Plant Cell Rep. 1991 Sep;10(6-7):354-7. doi: 10.1007/BF00193158.
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Virulence of Agrobacterium tumefaciens Strain A281 on Legumes.根癌土壤杆菌A281菌株对豆科植物的毒力
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T-DNA and opine synthetic loci in tumors incited by Agrobacterium tumefaciens A281 on soybean and alfalfa plants.根癌土壤杆菌A281在大豆和苜蓿植株上诱发的肿瘤中的Ti质粒DNA和冠瘿碱合成位点
番茄的病毒与植物寄生线虫:相互作用研究及可持续防治中的生物技术方法
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Efficient transgenic plant regeneration throughAgrobacterium-mediated transformation of Chickpea (Cicer arietinum L.).通过农杆菌介导的鹰嘴豆(Cicer arietinum L.)转化实现高效的转基因植物再生。
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
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A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.一种用于检测溶菌碱和胭脂碱脱氢酶活性的快速微量方法。
Biochim Biophys Acta. 1978 Dec 8;527(2):497-500. doi: 10.1016/0005-2744(78)90363-7.