Schneck Anne-Sophie, Iannelli Antonio, Patouraux Stéphanie, Rousseau Déborah, Bonnafous Stéphanie, Bailly-Maitre Beatrice, Le Thuc Ophélia, Rovere Carole, Panaia-Ferrari Patricia, Anty Rodolphe, Tran Albert, Gual Philippe, Gugenheim Jean
Centre Hospitalier Universitaire de Nice, Hôpital de l'Archet, Pôle Digestif, Nice, France,
Surg Endosc. 2014 Feb;28(2):592-602. doi: 10.1007/s00464-013-3211-1. Epub 2013 Oct 3.
Sleeve gastrectomy (SG) has become a popular bariatric procedure. The mechanisms responsible for weight loss and improvement of metabolic disturbances have still not been completely elucidated. We investigated the effect of SG on body weight, adipose tissue depots, glucose tolerance, and liver steatosis independent of reduced caloric intake in high-fat-diet-induced obese mice.
C57BI/6 J mice fed a high fat diet (45 %) for 33 weeks were divided into three groups: sleeve gastrectomy (SG, 13 mice), sham-operated ad libitum fed (SALF, 13 mice) and sham-operated pair fed (PFS, 13 mice). The animals were humanely killed 23 days after surgery.
In SG mice, food intake was reduced transiently, but weight loss was significant and persistent compared to controls (SG vs. PFS, P < 0.05; PFS vs. SALF, P < 0.05). SG mice showed improved glucose tolerance and lower levels of liver steatosis compared with controls (area under the curve, SG vs. PFS, P < 0.01; PFS vs. SALF, P < 0.05) (liver steatosis, SG vs. PFS, P < 0.05; PFS vs. SALF, P < 0.01). This was associated with a decrease in the ratios of the weight of pancreas, epididymal and inguinal adipose tissues to body weight, and an increase in the ratio of brown adipose tissue weight to body weight. Epididymal adipose tissue was also infiltrated by fewer activated T cells and by more anti-inflammatory regulatory T cells. Serum levels of fasting acyl ghrelin were still significantly decreased 3 weeks after surgery in SG mice compared to PFS mice (P < 0.05).
Reduced white adipose tissue inflammation, modification of adipose tissue development (brown vs. white adipose tissue), and ectopic fat are potential mechanisms that may account for the reduced caloric intake independent effects of SG.
袖状胃切除术（SG）已成为一种流行的减肥手术。导致体重减轻和代谢紊乱改善的机制尚未完全阐明。我们研究了SG对高脂饮食诱导的肥胖小鼠体重、脂肪组织库、葡萄糖耐量和肝脂肪变性的影响，且该影响独立于热量摄入的减少。
将喂食高脂饮食（45%）33周的C57BI/6 J小鼠分为三组：袖状胃切除术组（SG，13只小鼠）、假手术自由摄食组（SALF，13只小鼠）和假手术配对喂食组（PFS，13只小鼠）。术后23天对动物实施安乐死。
在SG小鼠中，食物摄入量短暂减少，但与对照组相比，体重减轻显著且持续（SG与PFS相比，P<0.05；PFS与SALF相比，P<0.05）。与对照组相比，SG小鼠的葡萄糖耐量得到改善，肝脂肪变性水平降低（曲线下面积，SG与PFS相比，P<0.01；PFS与SALF相比，P<0.05）（肝脂肪变性，SG与PFS相比，P<0.05；PFS与SALF相比，P<0.01）。这与胰腺、附睾和腹股沟脂肪组织重量与体重之比的降低以及棕色脂肪组织重量与体重之比的增加有关。附睾脂肪组织中活化T细胞浸润较少，抗炎调节性T细胞较多。与PFS小鼠相比，SG小鼠术后3周空腹酰基胃泌素血清水平仍显著降低（P<0.05）。
白色脂肪组织炎症减轻、脂肪组织发育改变（棕色与白色脂肪组织）和异位脂肪可能是导致SG独立于热量摄入减少作用的潜在机制。
