Department of Molecular Plant Physiology, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands.
Planta. 1990 Sep;182(2):298-304. doi: 10.1007/BF00197125.
Homogeneous populations of developing microspores and pollen from anthers of lily (Lilium longiflorum Thumb.) and tobacco (Nicotiana tabacum L.) show a continuous production of biomass, reaching a maximum in young pollen. The rate of RNA synthesis was 460 fg · h(-1) in young binucleate cells, 138 fg · h(-1) in late binucleate cells and 56 fg · h(-1) in microspores. The mRNA population in developing pollen can be separated into three groups. In the first group, certain types of mRNAs are present at a constant level during all stages of development. A second group is characteristic of young pollen and increases quantitatively until anthesis. A third group is seen transiently; to this belong mRNAs present only before mitosis or at a distinct cell stage after mitosis. Some of the translation products of this latter group of mRNAs showed similarities between lily and tobacco on two-dimensional gels in respect of molecular weight and isolectric point, indicating that those mRNAs and proteins play a role in the regulation of pollen development.
发育中的百合（百合属）和烟草（烟草属）小孢子和花粉的同质群体表现出持续的生物量产生，在幼花粉中达到最大值。年轻双核细胞中的 RNA 合成率为 460 fg·h(-1)，晚期双核细胞中的 RNA 合成率为 138 fg·h(-1)，小孢子中的 RNA 合成率为 56 fg·h(-1)。发育中的花粉中的 mRNA 群体可以分为三组。在第一组中，某些类型的 mRNA 在发育的所有阶段都保持恒定水平。第二组是幼花粉的特征，数量增加直到开花。第三组是短暂出现的；属于这一组的 mRNAs 仅在有丝分裂之前或有丝分裂后特定的细胞阶段存在。该组 mRNAs 的某些翻译产物在二维凝胶上显示出百合和烟草之间在分子量和等电点方面的相似性，表明这些 mRNAs 和蛋白质在花粉发育的调节中发挥作用。
