Department of Physiology and Biochemistry of Plants, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands.
Plant Cell Rep. 1993 May;12(7-8):474-7. doi: 10.1007/BF00234715.
Hairy root cultures were induced from leaf explants of Linum flavum by infection with Agrobacterium rhizogenes. The transformed nature of tissue was confirmed by the production of opines. The cultures produced 1.5 to 3.5% of the lignan 5-methoxypodophyllotoxin (5-MPT) on a dry weight basis, which was 2 to 5 times higher than the 5-MPT content in untransformed root cultures and 5 to 12 times higher than in L. flavum cell suspensions. The 5-MPT production as a function of time was up to four times higher than that in cell suspensions.
发根培养物是通过感染根癌农杆菌从亚麻黄花叶外植体诱导产生的。组织的转化性质通过产生冠瘿碱得到证实。培养物以干重为基础产生 1.5%至 3.5%的木质素 5-甲氧基足叶乙素（5-MPT），比未转化的根培养物中的 5-MPT 含量高 2 至 5 倍，比亚麻黄花细胞悬浮液中的 5-MPT 含量高 5 至 12 倍。5-MPT 的产生与时间的函数关系比细胞悬浮液中的高 4 倍。
