[高效液相色谱法同时测定冬凌草中迷迭香酸、冬凌草甲素和金荞麦黄酮]

[Simultaneous determination of rosmarinic acid, oridonin and chrysoplenetin in Isodon rubescens by HPLC].

作者信息

Yuan Xing-Li, Yan Li-Hua, Zhang Qi-Wei, Wang Zhi-Min

机构信息

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(14):2343-7.

PMID:24199569

Abstract

An HPLC method was developed for simultaneous quantitation of rosmarinic acid, oridonin and chrysoplenetin in the aerial parts of Isodon rubescens. Samples were analyzed on an Ultimate C18 column (4.6 mm x 250 mm, 5 microm) with methanol and water containing 0.1% formic acid as mobile phases in a linear gradient mode. The flow rate was 1.0 mL x min(-1) and the temperature was set at 30 degrees C. The PDA detector wavelengths were set at 338 nm for rosmarinic and chrysoplenetin and at 242 nm for oridonin. The linear ranges were 0.222-2.78, 0.227-2.84 and 0.005-0.071 microg for rosmarinic acid, oridonin and chrysoplenetin, respectively. The average recoveries of the three constituents were 102.9% (RSD 1.9%), 99.6% (RSD 1.1%) and 102.5% (RSD 0.94%), respectively. This method was proved to be accurate and repeatable, and can be used for quality control of the aerial parts of I. rubescens.

摘要

建立了一种高效液相色谱法，用于同时定量测定冬凌草地上部分中的迷迭香酸、冬凌草甲素和毛萼晶甲素。样品在Ultimate C18柱（4.6 mm×250 mm，5微米）上进行分析，以甲醇和含0.1%甲酸的水作为流动相，采用线性梯度模式。流速为1.0 mL·min⁻¹，温度设定为30℃。二极管阵列检测器（PDA）的波长，迷迭香酸和毛萼晶甲素设定为338 nm，冬凌草甲素设定为242 nm。迷迭香酸、冬凌草甲素和毛萼晶甲素的线性范围分别为0.222 - 2.78、0.227 - 2.84和0.005 - 0.071微克。三种成分的平均回收率分别为102.9%（相对标准偏差1.9%）、99.6%（相对标准偏差1.1%）和102.5%（相对标准偏差0.94%）。该方法经证明准确且可重复，可用于冬凌草地上部分的质量控制。