Department of Chemistry and ‡Deptartment of Biomedical Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
J Am Chem Soc. 2013 Nov 20;135(46):17290-3. doi: 10.1021/ja409378f. Epub 2013 Nov 11.
Surface-enhanced spatially offset Raman spectroscopy (SESORS) is a label-free vibrational spectroscopy that has the potential for in vivo imaging. Previous SESORS experiments have been limited to acquiring spectra using SERS substrates implanted under the skin or from nanoparticles embedded in tissue. Here we present SESORS measurements of SERS active nanoparticles coated with a Raman reporter molecule (nanotags) acquired, for the first time, through bone. We demonstrate the ability of SESORS to measure spectra through various thicknesses (3-8 mm) of bone. We also show that diluted nanotag samples (~2 × 10(12) particles) can be detected through the bone. We apply a least-squares support vector machine analysis to demonstrate quantitative detection. It is anticipated that these through-bone SESORS measurements will enable real-time, non-invasive spectroscopic measurement of neurochemicals through the skull, as well as other biomedical applications.
表面增强空间位移拉曼光谱（SESORS）是一种无标记的振动光谱技术，具有体内成像的潜力。以前的 SESORS 实验仅限于使用植入皮下的 SERS 基底或嵌入组织中的纳米粒子来获取光谱。在这里，我们首次通过骨骼介绍了涂有拉曼报告分子（纳米标签）的 SERS 活性纳米粒子的 SESORS 测量。我们证明了 SESORS 能够通过各种厚度（3-8 毫米）的骨骼测量光谱。我们还表明，稀释的纳米标签样品（~2×10(12)个粒子）可以通过骨骼检测到。我们应用最小二乘支持向量机分析来证明定量检测。预计这些穿透骨骼的 SESORS 测量将能够通过颅骨实时、非侵入性地测量神经化学物质，以及其他生物医学应用。
