Department of Chemistry, University of Tennessee Knoxville , 1420 Circle Drive, Knoxville, Tennessee 37996, United States.
Department of Chemistry, University of Virginia-Wise , 1 College Avenue, Wise, Virginia 24293, United States.
Anal Chem. 2017 Jun 6;89(11):5688-5692. doi: 10.1021/acs.analchem.7b00985. Epub 2017 May 16.
The ability to noninvasively detect neurotransmitters through the skull would aid in understanding brain function and the development of neurological diseases. Surface enhanced spatially offset Raman spectroscopy (SESORS) is a powerful technique that combines the sensitivity of surface-enhanced Raman spectroscopy (SERS) with the ability of spatially offset Raman spectroscopy (SORS) to probe subsurface layers. Here we present SERS measurements of neurotransmitters (melatonin, serotonin, and epinephrine) at various concentrations followed by the SESORS measurements of the neurotransmitters to a concentration as low as 100 μM in a brain tissue mimic through a cat skull. Principal components analysis was performed to distinguish between the surface bone layer and the subsurface layer, comprised of a brain tissue mimic modified with neurotransmitters, and to determine if each individual neurotransmitter could be accurately identified.
通过颅骨非侵入式检测神经递质的能力将有助于理解大脑功能和神经疾病的发展。表面增强空间偏移拉曼光谱（SESORS）是一种强大的技术，它结合了表面增强拉曼光谱（SERS）的灵敏度和空间偏移拉曼光谱（SORS）的能力，可探测亚表面层。在这里，我们展示了在不同浓度下对神经递质（褪黑素、血清素和肾上腺素）的 SERS 测量，然后通过猫颅骨对神经递质进行 SESORS 测量，在脑组织模拟物中的浓度低至 100 μM。进行主成分分析以区分表面骨层和亚表面层，亚表面层由用神经递质修饰的脑组织模拟物组成，并确定是否可以准确识别每个单独的神经递质。
