Gualberto J M, Wintz H, Weil J H, Grienenberger J M
Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
Mol Gen Genet. 1988 Dec;215(1):118-27. doi: 10.1007/BF00331312.
A region of about 2 kb which is almost identical in the wheat and maize mitochondrial genomes has been sequenced. It contains a tRNA(Ser) gene, a pseudo-tRNA gene and two open reading frames coding for subunit 3 of the NADH dehydrogenase (118 amino acids) and for ribosomal protein S12 (125 amino acids). The two protein genes are separated by 47 bp and are co-transcribed in wheat and maize. Two transcripts of about 0.9 kb and 3.0 kb, each coding for both proteins, have been characterized, but no monocistronic transcript was detected. Each gene is preceded by a putative ribosome binding site. The pseudo-tRNA gene is interrupted by two insertion sequences in wheat and by one in maize. The origin of the additional interrupting sequence found in the wheat pseudo-tRNA gene, which is also present elsewhere in the mitochondrial genomes, is discussed.
已对小麦和玉米线粒体基因组中一段长度约为2 kb且几乎完全相同的区域进行了测序。它包含一个tRNA(Ser)基因、一个假tRNA基因以及两个开放阅读框，分别编码NADH脱氢酶亚基3（118个氨基酸）和核糖体蛋白S12（125个氨基酸）。这两个蛋白质基因相隔47 bp，在小麦和玉米中共同转录。已鉴定出两条分别约为0.9 kb和3.0 kb的转录本，每个转录本都编码这两种蛋白质，但未检测到单顺反子转录本。每个基因之前都有一个假定的核糖体结合位点。假tRNA基因在小麦中被两个插入序列打断，在玉米中被一个插入序列打断。文中讨论了在小麦假tRNA基因中发现的额外打断序列的起源，该序列也存在于线粒体基因组的其他地方。
