Healicon R M, Foreman J C
Immunology. 1986 Apr;57(4):611-5.
The relationship between the valency of a ligand and the subsequent characteristics of histamine release was investigated in rat peritoneal mast cells. The cells were passively sensitized to the DNP hapten and a series of DNP-human serum albumin conjugates of known valency were used to induce histamine release. The rate of release of histamine induced by these conjugates was independent of the DNP/HSA ratio when the ratio was between 71.3 and 7.2. Marked slowing of the release occurred as the ratio was reduced below 7.2. The rate of desensitization of the cells slowed as a continuous function as the DNP/HSA ratio was reduced. 45Calcium uptake measurements showed that the changes in histamine release were paralleled by changes in the membrane permeability to calcium. The rate of release of histamine from mast cells and the rate of desensitization of the cells are discussed in terms of the size of IgE receptor complexes on the cell membrane.
在大鼠腹膜肥大细胞中研究了配体的化合价与随后组胺释放特性之间的关系。细胞被被动致敏于二硝基苯（DNP）半抗原，并使用一系列已知化合价的DNP-人血清白蛋白缀合物来诱导组胺释放。当这些缀合物的DNP/人血清白蛋白（HSA）比率在71.3和7.2之间时，它们诱导的组胺释放速率与DNP/HSA比率无关。当比率降至7.2以下时，释放明显减慢。随着DNP/HSA比率降低，细胞脱敏速率作为连续函数而减慢。45钙摄取测量表明，组胺释放的变化与细胞膜对钙的通透性变化平行。根据细胞膜上IgE受体复合物的大小讨论了肥大细胞中组胺的释放速率和细胞的脱敏速率。
