Weyer A, Dandeu J P, Etiévant M, Bourgeois J P, Marchand F, Prouvost-Danon A, David B
Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
Agents Actions. 1989 Apr;27(1-2):52-4. doi: 10.1007/BF02222196.
FPLC purification of mouse monoclonal anti-human IgE antibody xb6-16 showed 2 major peaks of different molecular weight, peak 1 (greater than 10(6) d) and peak 3 (1.6 x 10(5) d). Peak 1 consisted of IgG1 and IgM, peak 3 of IgG1 only. On a protein weight basis, peak 1 was 100 times more potent than peak 3 in inducing histamine release from human basophils. Preincubation of peak 3 with anti-IgG1 enhanced the mediator release triggered by this fraction. On this basis, the potentiating effect of aggregated IgG1 or IgG1-IgM complexes on mediator release from basophils is discussed.
通过快速蛋白质液相色谱法（FPLC）对小鼠单克隆抗人IgE抗体xb6 - 16进行纯化，结果显示出两个不同分子量的主要峰，峰1（大于10⁶ d）和峰3（1.6×10⁵ d）。峰1由IgG1和IgM组成，峰3仅由IgG1组成。以蛋白质重量为基础，峰1在诱导人嗜碱性粒细胞释放组胺方面的效力比峰3强100倍。将峰3与抗IgG1预温育可增强该组分引发的介质释放。在此基础上，讨论了聚集的IgG1或IgG1 - IgM复合物对嗜碱性粒细胞介质释放的增强作用。
