"G.P. Livanos and M. Simou Laboratories", Evangelismos Hospital, Department of Critical Care and Pulmonary Services, School of Medicine, University of Athens, 3 Ploutarchou Street, 10675 Athens, Greece.
Cancers (Basel). 2011 Feb 23;3(1):789-801. doi: 10.3390/cancers3010789.
Mastic oil from Pistacia lentiscus variation chia, a natural combination of bioactive terpenes, has been shown to exert anti-tumor growth effects against a broad spectrum of cancers including mouse Lewis lung adenocarcinomas (LLC). However, no studies have addressed its anti-metastatic actions. In this study, we showed that treatment of LLC cells with mastic oil within a range of non-toxic concentrations (0.01-0.04% v/v): (a) abrogated their Matrigel invasion and migration capabilities in transwell assays; (b) reduced the levels of secreted MMP-2; (c) restricted phorbol ester-induced actin remodeling and (d) limited the length of neo-vessel networks in tumor microenvironment in the model of chick embryo chorioallantoic membrane. Moreover, exposure of LLC and endothelial cells to mastic oil impaired their adhesive interactions in a co-culture assay and reduced the expression of key adhesion molecules by endothelial cells upon their stimulation with tumor necrosis factor-alpha. Overall, this study provides novel evidence supporting a multipotent role for mastic oil in prevention of crucial processes related to cancer metastasis.
乳香精油来自乳香黄连木变种，是生物活性萜烯的天然组合，已被证明对包括小鼠 Lewis 肺腺癌（LLC）在内的多种癌症具有抗肿瘤生长作用。然而，尚无研究探讨其抗转移作用。在这项研究中，我们表明，在无毒浓度范围内（0.01-0.04%v/v）用乳香精油处理 LLC 细胞：（a）在 Transwell 测定中消除了它们的 Matrigel 侵袭和迁移能力；（b）降低了分泌型 MMP-2 的水平；（c）限制了佛波酯诱导的肌动蛋白重塑；（d）在鸡胚绒毛尿囊膜模型中限制了肿瘤微环境中新血管网络的长度。此外，将 LLC 和内皮细胞暴露于乳香精油会损害它们在共培养测定中的粘附相互作用，并降低内皮细胞在受到肿瘤坏死因子-α刺激时关键粘附分子的表达。总的来说，这项研究提供了新的证据，支持乳香精油在预防与癌症转移相关的关键过程中的多效作用。
