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M1 RNA，即大肠杆菌核糖核酸酶P的RNA亚基，可发生pH敏感的构象变化。

M1 RNA, the RNA subunit of Escherichia coli ribonuclease P, can undergo a pH-sensitive conformational change.

作者信息

Altman S, Guerrier-Takada C

出版信息

Biochemistry. 1986 Mar 25;25(6):1205-8. doi: 10.1021/bi00354a002.

DOI:10.1021/bi00354a002
PMID:2421764
Abstract

After purification from extracts of whole cells, M1 RNA, the catalytic subunit of ribonuclease P from Escherichia coli, apparently must undergo a change in conformation before it can function catalytically. The rate of this conformational change is dependent upon the duration of incubation at various temperatures and pH. delta E of the transition at pH 7.5 is approximately 36 kcal/mol. The change in conformation is not sensitive to Mg2+ concentration between 10 and 100 mM. A decrease in A260 of M1 RNA in solution has been observed during the incubation period that potentiates the conformational change at 30 degrees C, but no direct correlation can yet be made to specific structural rearrangements.

摘要

从全细胞提取物中纯化后，来自大肠杆菌的核糖核酸酶P的催化亚基M1 RNA在能够进行催化功能之前，显然必须经历构象变化。这种构象变化的速率取决于在不同温度和pH下的孵育时间。在pH 7.5时转变的ΔE约为36千卡/摩尔。构象变化对10至100 mM之间的Mg2+浓度不敏感。在孵育期间观察到溶液中M1 RNA的A260降低，这增强了30℃下的构象变化，但尚未能与特定的结构重排建立直接关联。

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M1 RNA, the RNA subunit of Escherichia coli ribonuclease P, can undergo a pH-sensitive conformational change.M1 RNA，即大肠杆菌核糖核酸酶P的RNA亚基，可发生pH敏感的构象变化。
Biochemistry. 1986 Mar 25;25(6):1205-8. doi: 10.1021/bi00354a002.
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