从雄性可育甜菜线粒体中分离出的DNA小环的DNA序列及转录
DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugar beet mitochondria.
作者信息
Hansen B M, Marcker K A
出版信息
Nucleic Acids Res. 1984 Jun 11;12(11):4747-56. doi: 10.1093/nar/12.11.4747.
A minicircle consisting of 1440 bp was isolated from male fertile sugar beet mitochondria. The entire nucleotide sequence of the minicircle was determined. There are several stop codons in all three reading frames and it is therefore unlikely that the minicircle codes for a polypeptide product. However, the minicircle is transcribed yielding a major RNA product consisting of 850 bases in addition to two very minor RNA products of 1100 and 950 bases, respectively. All three RNA products are transcribed from the same strand. The starting point for transcription of the major RNA product was determined by S1 nuclease protection analysis.
从雄性可育甜菜线粒体中分离出一个由1440个碱基对组成的小环。测定了该小环的完整核苷酸序列。在所有三个阅读框中都有几个终止密码子，因此该小环不太可能编码一种多肽产物。然而，该小环被转录，除了分别由1100个碱基和950个碱基组成的两个非常小的RNA产物外，还产生了一个由850个碱基组成的主要RNA产物。所有这三种RNA产物都是从同一条链转录而来的。主要RNA产物的转录起始点通过S1核酸酶保护分析确定。
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