Strauss R G, Goeken J A, Imig K M, Greazel C
Transfusion. 1986 May-Jun;26(3):265-8. doi: 10.1046/j.1537-2995.1986.26386209384.x.
Immunologic studies were performed immediately before and after leukapheresis in five subjects undergoing four granulocyte donations (separated by 3- to 4-week intervals) using continuous-flow centrifugation leukapheresis with a rapidly excreted investigational analog of hydroxyethyl starch. Postleukapheresis donor blood lymphocyte counts and serum immunoglobulin levels decreased transiently, but significantly, during each procedure when compared with preleukapheresis values due simply to plasma volume expansion (acute effects of dilution). Possible long-term effects of repeated procedures were investigated by comparing preleukapheresis values before the first and fourth procedures. Total lymphocyte, total T-lymphocyte, and T4-lymphocyte counts, as well as serum immunoglobulin values and anti-A and B titers measured before the fourth procedure remained unchanged when compared with values obtained before beginning the series of procedures. In contrast, the B-lymphocyte count was decreased, the T8-lymphocyte count was increased, and the T4:T8 ratio was decreased due to the increase in the T8-lymphocyte count. Although statistically significant (p less than 0.05), all changes were slight, and clinically significant effects on donor humoral immunity could not be detected. The mechanisms involved, however, may be of biologic and therapeutic interest.
对5名接受4次粒细胞采集（间隔3至4周）的受试者，在白细胞单采术前后立即进行了免疫学研究，采用连续流动离心白细胞单采术，并使用一种快速排泄的羟乙基淀粉研究类似物。与白细胞单采术前的值相比，每次操作过程中白细胞单采术后供体血液淋巴细胞计数和血清免疫球蛋白水平短暂但显著下降，这仅仅是由于血浆量扩张（稀释的急性效应）。通过比较第一次和第四次操作前的白细胞单采术前值，研究了重复操作可能产生的长期影响。与开始一系列操作前获得的值相比，第四次操作前测量的总淋巴细胞、总T淋巴细胞和T4淋巴细胞计数，以及血清免疫球蛋白值和抗A和抗B滴度均保持不变。相比之下，B淋巴细胞计数下降，T8淋巴细胞计数增加，由于T8淋巴细胞计数增加，T4:T8比值下降。尽管具有统计学意义（p小于0.05），但所有变化都很轻微，未检测到对供体体液免疫有临床显著影响。然而，其中涉及的机制可能具有生物学和治疗学意义。
