Chen Xiaodong, Shi Yan, Shu Bin, Xie Xiaoxia, Yang Ronghua, Zhang Lijun, Ruan Shubin, Lin Yan, Lin Zepeng, Shen Rui, Zhang Fenggang, Feng Xiangsheng, Xie Julin
Department of Burn Surgery, Fo Shan Hospital of Sun Yat-Sen University Foshan, Guangdong, China.
Int J Clin Exp Pathol. 2013 Oct 15;6(11):2280-91. eCollection 2013.
To study the effect of porcine acellular dermal matrix (ADM) on the burn wound healing. Seventy healthy Wistar rats were inflicted with 2 cm second degree burn and divided into 2 groups; one group was treated with porcine ADM and the other with Povidone Iodine Cream. Biopsies were taken on day 1, 3, 5, 7, 10, 14, 21 for histopathological and biochemical analysis to test PCNA, K19, Integrin-β1, PDGF, EGF and FGF. The results revealed relatively better and faster regeneration after treatment of porcine ADM, along with greatly increased synthesis in collagen in the experimental group. PCNA, K19, Integrin-β1 had an increase and then tapered down, and were stronger in the experimental group than in the contrast group during 21 days after burns. PDGF, EGF and FGF levels increased on day 3, peaked on day 5 and then started to decrease, while significantly enhanced expression of relevant growth factors were observed in the experimental group. Porcine ADM stimulate collagen synthesis, stem cells proliferation and differentiation, and the expression of relevant growth factors and ultimately improve the burn wound healing.
研究猪脱细胞真皮基质（ADM）对烧伤创面愈合的影响。将70只健康的Wistar大鼠造成2厘米深二度烧伤，并分为2组；一组用猪ADM治疗，另一组用聚维酮碘乳膏治疗。在第1、3、5、7、10、14、21天取活检组织进行组织病理学和生化分析，检测增殖细胞核抗原（PCNA）、细胞角蛋白19（K19）、整合素β1（Integrin-β1）、血小板衍生生长因子（PDGF）、表皮生长因子（EGF）和成纤维细胞生长因子（FGF）。结果显示，猪ADM治疗后再生相对更好、更快，实验组胶原蛋白合成大幅增加。PCNA、K19、Integrin-β1先升高后下降，烧伤后21天内实验组高于对照组。PDGF、EGF和FGF水平在第3天升高，第5天达到峰值，然后开始下降，而实验组相关生长因子表达明显增强。猪ADM刺激胶原蛋白合成、干细胞增殖分化及相关生长因子表达，最终促进烧伤创面愈合。
