Rebolledo-Mendez Jovan D, Vaishnav Radhika A, Cooper Nigel G, Friedland Robert P
Department of Biochemistry and Molecular Biology; University of Louisville, KY USA.
Commun Integr Biol. 2013 Sep 1;6(5):e24951. doi: 10.4161/cib.24951. Epub 2013 May 21.
Micro-RNAs regulate the expression of cellular and tissue phenotypes at a post-transcriptional level through a complex process involving complementary interactions between micro-RNAs and messenger-RNAs. Similar nucleotide interactions have been shown to occur as cross-kingdom events; for example, between plant viruses and plant micro-RNAs and also between animal viruses and animal micro-RNAs. In this study, this view is expanded to look for cross-kingdom similarities between plant virus and human micro-RNA sequences. A method to identify significant nucleotoide sequence similarities between plant viruses and hsa micro-RNAs was created. Initial analyses demonstrate that plant viruses contain nucleotide sequences which exactly match the seed sequences of human micro-RNAs in both parallel and anti-parallel directions. For example, the bean common mosaic virus strain NL4 from Colombia contains sequences that match exactly the seed sequence for micro-RNA of the hsa-mir-1226 in the parallel direction, which suggests a cross-kingdom conservation. Similarly, the rice yellow stunt viral cRNA contains a sequence that is an exact match in the anti-parallel direction to the seed sequence of hsa-micro-RNA let-7b. The functional implications of these results need to be explored. The finding of these cross-kingdom sequence similarities is a useful starting point in support of bench level investigations.
微小RNA通过一个复杂过程在转录后水平调节细胞和组织表型的表达，该过程涉及微小RNA与信使RNA之间的互补相互作用。类似的核苷酸相互作用已被证明是跨物种事件；例如，在植物病毒与植物微小RNA之间以及动物病毒与动物微小RNA之间。在本研究中，这一观点得到扩展，以寻找植物病毒与人类微小RNA序列之间的跨物种相似性。创建了一种鉴定植物病毒与人类微小RNA之间显著核苷酸序列相似性的方法。初步分析表明，植物病毒含有在平行和反平行方向上与人类微小RNA种子序列完全匹配的核苷酸序列。例如，来自哥伦比亚的菜豆普通花叶病毒株系NL4含有与hsa-mir-1226微小RNA种子序列在平行方向上完全匹配的序列，这表明存在跨物种保守性。同样，水稻黄矮病毒cRNA含有一个与hsa-微小RNA let-7b种子序列在反平行方向上完全匹配的序列。这些结果的功能意义有待探索。这些跨物种序列相似性的发现是支持实验室水平研究的一个有用起点。
