Arch Virol. 2014 May;159(5):1175-80. doi: 10.1007/s00705-013-1901-6.
To evaluate the prevalence and genetic diversity of porcine sapeloviruses (PSVs) in Korea, a total of 100 diarrhea fecal samples from pigs were analyzed by RT-PCR and nested PCR assays with primer pairs specific for the VP1 gene. Overall, 34 % of the diarrhea samples tested positive for PSV, and a high proportion of infections occurred along with a variety of other enteric viruses and bacteria. Genomic and phylogenetic analysis of the VP1 genes revealed pronounced genetic diversities between PSVs from Korean and elsewhere. Our results indicate that PSV infections are very common in Korean pigs with diarrhea. The infecting strains are genetically diverse.
为了评估韩国猪细小病毒(PSV)的流行情况和遗传多样性,我们使用针对 VP1 基因的 RT-PCR 和巢式 PCR 引物对分析了来自 100 份腹泻粪便样本。总的来说,34%的腹泻样本检测到 PSV 阳性,并且感染比例较高,同时还伴随着多种其他肠病毒和细菌。VP1 基因的基因组和系统发育分析表明,韩国和其他地区的 PSV 之间存在明显的遗传多样性。我们的结果表明,PSV 感染在韩国腹泻猪中非常普遍。感染株具有遗传多样性。