Bak Geon-Yong, Kang Mun-Il, Son Kyu-Yeol, Park Jun-Gyu, Kim Deok-Song, Seo Ja-Young, Kim Ji-Yun, Alfajaro Mia Madel, Soliman Mahmoud, Baek Yeong-Bin, Cho Eun-Hyo, Kwon Joseph, Choi Jong-Soon, Park Sang-Ik, Cho Kyoung-Oh
Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
J Vet Med Sci. 2017 Jan 10;78(12):1911-1914. doi: 10.1292/jvms.16-0237. Epub 2016 Sep 11.
To determine the occurrence and genetic diversity of Sapelovirus A (SV-A) in diarrhea and non-diarrhea feces of Korean pigs, 110 specimens from different age groups of pigs in the same farm were analyzed by RT-nested PCR. SV-As were detected in 60% of both diarrhea and non-diarrhea specimens regardless of age groups with primer pairs for 2C region, in which all diarrhea samples were co-infected by other enteric pathogens. Phylogenetical analysis of partial VP1 region showed that our strains and several other Korean strains belonged to cluster I, distinct from some strains reported in Korea and other countries. These data indicate that genetically distinct SV-As are frequently detected in Korean pigs irrespective of diarrhea and age.
为了确定韩国猪腹泻和非腹泻粪便中萨佩洛病毒A（SV-A）的发生率和遗传多样性，对同一农场不同年龄组的110份猪样本进行了逆转录巢式聚合酶链反应（RT-nested PCR）分析。无论年龄组如何，使用针对2C区域的引物对，在60%的腹泻和非腹泻样本中均检测到了SV-A，其中所有腹泻样本均被其他肠道病原体共同感染。部分VP1区域的系统发育分析表明，我们的毒株和其他一些韩国毒株属于第一簇，与韩国和其他国家报道的一些毒株不同。这些数据表明，无论腹泻情况和年龄如何，在韩国猪中经常检测到基因不同的SV-A。
