Darlington Todd M, McCarthy Riley D, Cox Ryan J, Ehringer Marissa A
Institute for Behavioral Genetics, Department of Integrative Physiology, University of Colorado, Boulder, Boulder, CO 80303, USA.
Institute for Behavioral Genetics, Department of Integrative Physiology, University of Colorado, Boulder, Boulder, CO 80303, USA.
Behav Brain Res. 2014 Feb 1;259:313-20. doi: 10.1016/j.bbr.2013.11.011. Epub 2013 Nov 14.
The mesolimbic dopaminergic pathway has been implicated in many rewarding behaviors, including the consumption of ethanol and voluntary exercise. It has become apparent that different rewarding stimuli activate this pathway, and therefore it is possible for these behaviors to influence each other, i.e. hedonic substitution. Using adult female C57BL/6J mice, we demonstrate that voluntary access to a running wheel substantially reduces the consumption and preference of ethanol. Furthermore, we examined gene expression of several genes involved in regulating the mesolimbic dopaminergic pathway, which we hypothesized to be the main pathway involved in hedonic substitution. In the striatum, we observed a reduction in mRNA expression of Drd1a due to exercise. Hippocampal Bdnf mRNA increased in response to exercise and decreased in response to ethanol. Furthermore, there was an interaction effect of exercise and ethanol on the expression of Slc18a2 in the midbrain. These data suggest an important role for this pathway, and especially for Bdnf and Slc18a2 in regulating hedonic substitution.
中脑边缘多巴胺能通路与许多奖赏行为有关，包括乙醇摄入和自愿运动。很明显，不同的奖赏刺激会激活该通路，因此这些行为有可能相互影响，即享乐替代。我们使用成年雌性C57BL/6J小鼠证明，自愿使用跑步轮可显著减少乙醇的摄入量和偏好。此外，我们检测了几个参与调节中脑边缘多巴胺能通路的基因的表达，我们假设该通路是参与享乐替代的主要通路。在纹状体中，我们观察到由于运动导致Drd1a的mRNA表达降低。海马体中的Bdnf mRNA在运动时增加，在摄入乙醇时减少。此外，运动和乙醇对中脑Slc18a2的表达有交互作用。这些数据表明该通路，特别是Bdnf和Slc18a2在调节享乐替代中起重要作用。
